Date published: 2026-1-10

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RPA40 Antibody (H-7): sc-374444

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Datasheets
  • RPA40 Antibody (H-7) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
  • raised against amino acids 1-105 of RPA40 of human origin
  • recommended for detection of RPA40 of mouse, rat and human origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for RPA40 Antibody (H-7) for WB applications. These reagents are now offered in bundles with RPA40 Antibody (H-7) (see ordering information below).

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    RPA40 Antibody (H-7) is a mouse monoclonal IgG1 antibody that detects RPA40 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RPA40 is a critical subunit of RNA polymerase I (Pol I), which plays a vital role in ribosomal RNA synthesis and, consequently, ribosomal biogenesis. Located in the nucleolus, RPA40 is essential for the assembly and stability of the Pol I complex, interacting with other subunits like RPA16 and RPA135 during the early stages of RNA polymerase assembly. The proper functioning of RPA40 is crucial, as RPA40 not only supports Pol I activity but is also a key component of RNA polymerase III, which transcribes other essential RNA molecules. The intricate regulation of RPA40 and its interactions with various signaling pathways highlight RPA40′s importance in cellular growth and proliferation, making anti-RPA40 antibody (H-7) a significant tool for studies related to ribosome biogenesis and its implications in diseases such as cancer.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    RPA40 Antibody (H-7)

    sc-374444
    200 µg/ml
    $322.00

    RPA40 Antibody (H-7): m-IgG Fc BP-HRP Bundle

    sc-540455
    200 µg Ab; 10 µg BP
    $361.00

    RPA40 Antibody (H-7): m-IgG1 BP-HRP Bundle

    sc-542134
    200 µg Ab; 20 µg BP
    $361.00

    What is the recommended fixation method for immunofluorescence staining with RPA40 (H-7): sc-374444 monoclonal antibody?

    Asked by: cjMara
    Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 5 out of 5 by from excellent in WBWe detected positive signal in WB using K562 whole cell extract
    Date published: 2016-11-21
    Rated 5 out of 5 by from Signal via western blot analysis of RPA40Signal via western blot analysis of RPA40 expression in A-431 and K-562 whole cell lysates. -SCBT QC
    Date published: 2015-05-14
    Rated 5 out of 5 by from Signla via immunofluorescence staining of methanolSignla via immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar and perinuclear localization. -SCBT QC
    Date published: 2014-09-23
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    RPA40 Antibody (H-7) is rated 5.0 out of 5 by 3.
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