RPA40 Antibody (H-6) recommended for detection of RPA40 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

RPA40 Antibody (H-6): sc-374443

RPA40 Antibody (H-6) is rated 5.0 out of 5 by 4.
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Datasheets

    • Anti-RPA40 Antibody (H-6) is a mouse monoclonal IgG1 (kappa light chain) RPA40 antibody provided at 200 µg/ml
    • raised against amino acids 1-105 of RPA40 of human origin
    • Anti-RPA40 Antibody (H-6) is recommended for detection of RPA40 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-RPA40 Antibody (H-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of RPA40 (H-6): sc-374443.
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Can RPA40 (H-6): sc-374443 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, RPA40 (H-6): sc-374443 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Great! We bought it several months ago, works very well. The signal specifically localizes in the nucleolus.
Date published: 2017-07-10
Rated 5 out of 5 by from Signal via immunoperoxidase staining of formalin Signal via immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining of subset of glandular cells. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by from Signal via western blot analysis of RPA40 Signal via western blot analysis of RPA40 expression in A-431 and K-562 whole cell lysates. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by from Signal via immunofluorescence staining of methanol Signal via immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar localization. -SCBT QC
Date published: 2014-07-10
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