RNase L Antibody (E-9): sc-74405

RNase L Antibody (E-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects RNase L protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-RNase L antibody (E-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RNase L plays a crucial role in the antiviral and antiproliferative activities of interferons, functioning as a key component of the interferon-regulated 2-5A system. This system is vital for the cellular response to viral infections, as RNase L activation leads to viral RNA degradation, thereby inhibiting viral replication. Additionally, mutations in RNase L have been linked to an increased risk of prostate cancer, making RNase L a significant candidate for the hereditary prostate cancer 1 (HPC1) allele. RNase L expression increases following interferon treatment, which also stimulates the production of 5-prime-triphosphorylated, 2-prime,5-prime-oligoadenylates (2-5A) from ATP by a group of synthetases. The importance of the 2-5A system in regulating viral and cellular growth underscores how defects in RNase L could lead to compromised immunity against viral infections and increased cancer susceptibility.

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RNase L Antibody (E-9) References:

1. Ribonuclease H1 maps to chromosome 2 and has at least three pseudogene loci in the human genome.  |  ten Asbroek, AL., et al. 2002. Genomics. 79: 818-23. PMID: 12036296
2. Ribonuclease L proteolysis in peripheral blood mononuclear cells of chronic fatigue syndrome patients.  |  Demettre, E., et al. 2002. J Biol Chem. 277: 35746-51. PMID: 12118002
3. Implications for RNase L in prostate cancer biology.  |  Silverman, RH. 2003. Biochemistry. 42: 1805-12. PMID: 12590567
4. Role of genetic polymorphisms of the RNASEL gene on familial prostate cancer risk in a Japanese population.  |  Nakazato, H., et al. 2003. Br J Cancer. 89: 691-6. PMID: 12915880
5. An apoptotic signaling pathway in the interferon antiviral response mediated by RNase L and c-Jun NH2-terminal kinase.  |  Li, G., et al. 2004. J Biol Chem. 279: 1123-31. PMID: 14570908
6. RNase L restricts the mobility of engineered retrotransposons in cultured human cells.  |  Zhang, A., et al. 2014. Nucleic Acids Res. 42: 3803-20. PMID: 24371271
7. RNase L Reprograms Translation by Widespread mRNA Turnover Escaped by Antiviral mRNAs.  |  Burke, JM., et al. 2019. Mol Cell. 75: 1203-1217.e5. PMID: 31494035
8. RNase L Amplifies Interferon Signaling by Inducing Protein Kinase R-Mediated Antiviral Stress Granules.  |  Manivannan, P., et al. 2020. J Virol. 94: PMID: 32295917
9. Identification of Small Molecule Inhibitors of RNase L by Fragment-Based Drug Discovery.  |  Tang, J., et al. 2022. J Med Chem. 65: 1445-1457. PMID: 34841869
10. Recurrent viral capture of cellular phosphodiesterases that antagonize OAS-RNase L.  |  Goldstein, SA. and Elde, NC. 2024. Proc Natl Acad Sci U S A. 121: e2312691121. PMID: 38277437
11. Molecular cloning and expression of cDNA for human RNase H.  |  Wu, H., et al. 1998. Antisense Nucleic Acid Drug Dev. 8: 53-61. PMID: 9512096
12. Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase HI.  |  Cerritelli, SM. and Crouch, RJ. 1998. Genomics. 53: 300-7. PMID: 9799596

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RNase L Antibody (E-9) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-RNase L Antibody (E-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RNase L Antibody (E-9) Specifications

Alternate Product Name: RNase L

Product Clone Name: E-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 442-741

Epitope Target: RNase L

Epitope Species: human

Product Reactivity/Detection: RNase L

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for RNase L Antibody (E-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin