RNase H1 Antibody (D-3): sc-393802

RNase H1 Antibody (D-3) is a mouse monoclonal IgG2a antibody that detects RNase H1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RNase H1 is a crucial cytoplasmic endonuclease that specifically degrades the RNA strand of RNA-DNA hybrids, producing 5′-phosphomonoester products, which is vital for maintaining genomic integrity and facilitating proper DNA replication and repair processes. The activity of RNase H1 can be inhibited by agents such as manganese ions and N-ethylmaleimide, highlighting the importance of metal ion regulation in its function. Notably, RNase H1 is consistently expressed across various human tissues, suggesting its role as a housekeeping protein essential for cellular homeostasis. Mapping to chromosome 2p25.3, RNase H1 also has pseudogenes located on chromosomes 17p11.2 and 1q, indicating a complex evolutionary history that may influence its functional regulation. Anti-RNase H1 antibody (D-3) is an invaluable tool for researchers studying RNA metabolism and the molecular mechanisms underlying nucleic acid interactions.

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RNase H1 Antibody (D-3) References:

1. Properties of cloned and expressed human RNase H1.  |  Wu, H., et al. 1999. J Biol Chem. 274: 28270-8. PMID: 10497183
2. Selective inhibitory DNA aptamers of the human RNase H1.  |  Pileur, F., et al. 2003. Nucleic Acids Res. 31: 5776-88. PMID: 14500841
3. Human RNase H1 uses one tryptophan and two lysines to position the enzyme at the 3'-DNA/5'-RNA terminus of the heteroduplex substrate.  |  Lima, WF., et al. 2003. J Biol Chem. 278: 49860-7. PMID: 14506260
4. Structure of human RNase H1 complexed with an RNA/DNA hybrid: insight into HIV reverse transcription.  |  Nowotny, M., et al. 2007. Mol Cell. 28: 264-76. PMID: 17964265
5. Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif.  |  Nguyen, TN., et al. 2013. FEBS Lett. 587: 1418-23. PMID: 23523920
6. Antisense oligonucleotides capable of promoting specific target mRNA reduction via competing RNase H1-dependent and independent mechanisms.  |  Vickers, TA. and Crooke, ST. 2014. PLoS One. 9: e108625. PMID: 25299183
7. XRN2 is required for the degradation of target RNAs by RNase H1-dependent antisense oligonucleotides.  |  Hori, S., et al. 2015. Biochem Biophys Res Commun. 464: 506-11. PMID: 26159921
8. The rates of the major steps in the molecular mechanism of RNase H1-dependent antisense oligonucleotide induced degradation of RNA.  |  Vickers, TA. and Crooke, ST. 2015. Nucleic Acids Res. 43: 8955-63. PMID: 26384424
9. R-loops acted on by RNase H1 influence DNA replication timing and genome stability in Leishmania.  |  Damasceno, JD., et al. 2025. Nat Commun. 16: 1470. PMID: 39922816
10. RNase H1 can catalyze RNA/DNA hybrid formation and cleavage with stable hairpin or duplex DNA oligomers.  |  Li, J. and Wartell, RM. 1998. Biochemistry. 37: 5154-61. PMID: 9548746

The preferred RNase H1 antibody is RNase H1 Antibody (H-4): sc-376326.