RNase H1 Antibody (H-4): sc-376326

RNase H1 Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects RNase H1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). RNase H1 (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RNase H1 plays a crucial role in the degradation of RNA within RNA-DNA hybrids, which is essential for maintaining genomic integrity and facilitating proper DNA replication and repair processes. This endonuclease activity is particularly important in cellular responses to DNA damage, as RNase H1 helps prevent the accumulation of potentially harmful RNA-DNA hybrids that can interfere with normal cellular functions. The human RNase H1 gene is located on chromosome 2p25.3, with pseudogenes found on chromosomes 17p11.2 and 1q, and RNase H1 is expressed at consistent levels across various human tissues, suggesting a function as a housekeeping protein vital for cellular homeostasis.

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RNase H1 Antibody (H-4) References:

1. Investigating the structure of human RNase H1 by site-directed mutagenesis.  |  Wu, H., et al. 2001. J Biol Chem. 276: 23547-53. PMID: 11319219
2. Ribonuclease H1 maps to chromosome 2 and has at least three pseudogene loci in the human genome.  |  ten Asbroek, AL., et al. 2002. Genomics. 79: 818-23. PMID: 12036296
3. Human RNase H1 activity is regulated by a unique redox switch formed between adjacent cysteines.  |  Lima, WF., et al. 2003. J Biol Chem. 278: 14906-12. PMID: 12473655
4. Human RNase H1 uses one tryptophan and two lysines to position the enzyme at the 3'-DNA/5'-RNA terminus of the heteroduplex substrate.  |  Lima, WF., et al. 2003. J Biol Chem. 278: 49860-7. PMID: 14506260
5. Determination of the role of the human RNase H1 in the pharmacology of DNA-like antisense drugs.  |  Wu, H., et al. 2004. J Biol Chem. 279: 17181-9. PMID: 14960586
6. Structural requirements at the catalytic site of the heteroduplex substrate for human RNase H1 catalysis.  |  Lima, WF., et al. 2004. J Biol Chem. 279: 36317-26. PMID: 15205459
7. Structural basis of the RNase H1 activity on stereo regular borano phosphonate DNA/RNA hybrids.  |  Johnson, CN., et al. 2011. Biochemistry. 50: 3903-12. PMID: 21443203
8. Divalent metal ion-induced folding mechanism of RNase H1 from extreme halophilic archaeon Halobacterium sp. NRC-1.  |  Tannous, E. and Kanaya, S. 2014. PLoS One. 9: e109016. PMID: 25268753
9. Primer retention owing to the absence of RNase H1 is catastrophic for mitochondrial DNA replication.  |  Holmes, JB., et al. 2015. Proc Natl Acad Sci U S A. 112: 9334-9. PMID: 26162680
10. Dynamic nucleoplasmic and nucleolar localization of mammalian RNase H1 in response to RNAP I transcriptional R-loops.  |  Shen, W., et al. 2017. Nucleic Acids Res. 45: 10672-10692. PMID: 28977560
11. Molecular cloning and expression of cDNA for human RNase H.  |  Wu, H., et al. 1998. Antisense Nucleic Acid Drug Dev. 8: 53-61. PMID: 9512096
12. Cloning, expression, and mapping of ribonucleases H of human and mouse related to bacterial RNase HI.  |  Cerritelli, SM. and Crouch, RJ. 1998. Genomics. 53: 300-7. PMID: 9799596

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RNase H1 Antibody (H-4) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-RNase H1 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RNase H1 Antibody (H-4) Specifications

Alternate Product Name: RNase H1

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 241-275

Epitope Target: RNase H1

Epitope Species: human

Product Reactivity/Detection: RNase H1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for RNase H1 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin