RLF Antibody (D-7): sc-365373
- RLF Antibody (D-7) is a mouse monoclonal IgG2b κ RLF antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 7-33 near the N-terminus of RLF of human origin
- RLF Antibody (D-7) is recommended for detection of RLF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, bovine and porcine
- Anti-RLF Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for RLF Antibody (D-7) for WB and IHC(P) applications. These reagents are now offered in bundles with RLF Antibody (D-7) (see ordering information below).
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SEE ALSO...
RLF Antibody (D-7) is a mouse monoclonal IgG2b kappa light chain antibody that detects RLF protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). RLF (D-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RLF protein, also known as Ras-associated protein Rab2L or RalGDS-like factor, functions as a guanine nucleotide exchange factor (GEF) for the small GTPase Ral, playing a crucial role in the Ras signaling pathway. By facilitating the exchange of GDP for GTP on Ral, RLF is integral to the activation of downstream signaling cascades that regulate cellular processes such as proliferation and differentiation. RLF interacts with the GTP-bound form of Ras through its C-terminal Ras-binding domain, which is structurally similar to that of RalGDS. This interaction is essential for the phosphorylation of c-Jun at Serines 63 and 73, linking RLF to oncogenic signaling pathways. RLF′s ability to induce Ral activation and c-Fos promoter activity is enhanced when targeted to the plasma membrane, demonstrating RLF′s role in mediating distinct Ras-induced signaling pathways that promote gene induction and cell growth. Overexpression of RLF, particularly in its constitutively active form (RLF-CAAX), has been shown to stimulate neuroretina cell division without affecting ERK activity, highlighting RLF′s potential as a therapeutic target in conditions where Ras signaling is dysregulated.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
RLF Antibody (D-7) References:
- The human RGL (RalGDS-like) gene: cloning, expression analysis and genomic organization. | Sood, R., et al. 2000. Biochim Biophys Acta. 1491: 285-8. PMID: 10760592
- Induction of postmitotic neuroretina cell proliferation by distinct Ras downstream signaling pathways. | Peyssonnaux, C., et al. 2000. Mol Cell Biol. 20: 7068-79. PMID: 10982823
- Ras-dependent regulation of c-Jun phosphorylation is mediated by the Ral guanine nucleotide exchange factor-Ral pathway. | de Ruiter, ND., et al. 2000. Mol Cell Biol. 20: 8480-8. PMID: 11046144
- Structure determination of the Ras-binding domain of the Ral-specific guanine nucleotide exchange factor Rlf. | Esser, D., et al. 1998. Biochemistry. 37: 13453-62. PMID: 9753431
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
RLF Antibody (D-7) | sc-365373 | 200 µg/ml | $316.00 | |||
RLF Antibody (D-7): m-IgG Fc BP-HRP Bundle | sc-529362 | 200 µg Ab; 10 µg BP | $354.00 | |||
RLF Antibody (D-7): m-IgGκ BP-HRP Bundle | sc-522226 | 200 µg Ab, 40 µg BP | $354.00 | |||
RLF Antibody (D-7): m-IgG2b BP-HRP Bundle | sc-550097 | 200 µg Ab; 10 µg BP | $354.00 | |||
RLF Antibody (D-7) AC | sc-365373 AC | 500 µg/ml, 25% agarose | $416.00 | |||
RLF Antibody (D-7) HRP | sc-365373 HRP | 200 µg/ml | $316.00 | |||
RLF Antibody (D-7) FITC | sc-365373 FITC | 200 µg/ml | $330.00 | |||
RLF Antibody (D-7) PE | sc-365373 PE | 200 µg/ml | $343.00 | |||
RLF Antibody (D-7) Alexa Fluor® 488 | sc-365373 AF488 | 200 µg/ml | $357.00 | |||
RLF Antibody (D-7) Alexa Fluor® 546 | sc-365373 AF546 | 200 µg/ml | $357.00 | |||
RLF Antibody (D-7) Alexa Fluor® 594 | sc-365373 AF594 | 200 µg/ml | $357.00 | |||
RLF Antibody (D-7) Alexa Fluor® 647 | sc-365373 AF647 | 200 µg/ml | $357.00 | |||
RLF Antibody (D-7) Alexa Fluor® 680 | sc-365373 AF680 | 200 µg/ml | $357.00 | |||
RLF Antibody (D-7) Alexa Fluor® 790 | sc-365373 AF790 | 200 µg/ml | $357.00 | |||
RLF (D-7) Neutralizing Peptide | sc-365373 P | 100 µg/0.5 ml | $68.00 |