Ref-1 Antibody (H-6): sc-55498

Ref-1 Antibody (H-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects Ref-1 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Ref-1 antibody (H-6) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatility for different experimental needs. Ref-1, also known as redox factor 1, plays a crucial role in regulating transcription factors by modulating their redox state, which is essential for maintaining proper gene expression. Ref-1 (H-6) monoclonal antibody targets a protein primarily located in the nucleus, where Ref-1 participates in DNA repair processes and influences transcription factor activity by protecting critical cysteine residues from oxidative damage. Ref-1′s ability to restore DNA binding activity of transcription factors like Fos and Jun after oxidative stress highlights its importance in cellular responses to environmental changes and stressors. Understanding Ref-1′s function and localization helps researchers better appreciate its role in gene regulation and potential implications in diseases where redox balance is disrupted.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ref-1 Antibody (H-6) References:

1. Ref-1 and transcriptional control of endothelial apoptosis.  |  Wang, N. and Stemerman, MB. 2001. Circ Res. 88: 1223-5. PMID: 11420295
2. Dynamic Regulation of APE1/Ref-1 as a Therapeutic Target Protein.  |  Choi, S., et al. 2016. Chonnam Med J. 52: 75-80. PMID: 27231670
3. Inhibitors of nuclease and redox activity of apurinic/apyrimidinic endonuclease 1/redox effector factor 1 (APE1/Ref-1).  |  Laev, SS., et al. 2017. Bioorg Med Chem. 25: 2531-2544. PMID: 28161249
4. APE1/Ref-1 as a Novel Target for Retinal Diseases.  |  Heisel, C., et al. 2021. J Cell Signal. 2: 133-138. PMID: 34322687
5. New Horizons for the Roles and Association of APE1/Ref-1 and ABCA1 in Atherosclerosis.  |  Chen, W., et al. 2021. J Inflamm Res. 14: 5251-5271. PMID: 34703267
6. Pharmacological inhibition of Ref-1 enhances the therapeutic sensitivity of papillary thyroid carcinoma to vemurafenib.  |  Hu, L., et al. 2022. Cell Death Dis. 13: 124. PMID: 35136031
7. APE1/Ref-1 Inhibits Adipogenic Transcription Factors during Adipocyte Differentiation in 3T3-L1 Cells.  |  Lee, EO., et al. 2023. Int J Mol Sci. 24: PMID: 36834665
8. In vitro and In vivo evidence demonstrating chronic absence of Ref-1 Cysteine 65 impacts Ref-1 folding configuration, redox signaling, proliferation and metastasis in pancreatic cancer.  |  Mijit, M., et al. 2024. Redox Biol. 69: 102977. PMID: 38056311
9. Protective effect of secretory APE1/Ref-1 on doxorubicin-induced cardiotoxicity via suppression of ROS and p53 pathway.  |  An, SY., et al. 2024. ESC Heart Fail. 11: 1182-1193. PMID: 38286792
10. New Ref-1/APE1 targeted inhibitors demonstrating improved potency for clinical applications in multiple cancer types.  |  Gampala, S., et al. 2024. Pharmacol Res. 201: 107092. PMID: 38311014

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ref-1 Antibody (H-6) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-Ref-1 Antibody (H-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ref-1 Antibody (H-6) Specifications

Alternate Product Name: APEX1

Product Clone Name: H-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: Ref-1

Epitope Species: human

Product Reactivity/Detection: Ref-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for Ref-1 Antibody (H-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Ref-1 antibody is Ref-1 Antibody (C-4): sc-17774.