Anti-Ref-1 Antibody (C-4) is a mouse monoclonal IgG2b κ Ref-1 antibody, cited in 49 publications, provided at 200 µg/ml
raised against amino acids 1-300 of Ref-1 of human origin
Anti-Ref-1 Antibody (C-4) is recommended for detection of Ref-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
Anti-Ref-1 Antibody (C-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
TransCruz reagent for Gel Supershift and ChIP applications (sc-17774 X, 200 µg/0.1 ml)
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ref-1 (C-4): sc-17774.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for Ref-1 Antibody (C-4) for WB and IHC(P) applications. These reagents are now offered in bundles with Ref-1 Antibody (C-4) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Ref-1 Antibody (C-4) is a high quality monoclonal Ref-1 antibody (also designated apurinic/apyrimidinic (AP) endodeoxyribonuclease 1 antibody, APE1 antibody, apurinic/apyrimidinic (AP) activity DNA repair enzyme antibody, AP endonuclease (APEN) antibody, or DNA-(apurinic or apyrimidinic site) lyase antibody) suitable for the detection of the Ref-1 protein of mouse, rat and human origin. Ref-1 Antibody (C-4) is available as both the non-conjugated anti-Ref-1 antibody form, as well as multiple conjugated forms of anti-Ref-1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The role of transcription factors in the regulation of gene expression is well established. Although the activity of these factors can be regulated by phosphorylation, evidence has indicated regulation of DNA binding mediated by changes in reduction-oxidation (redox) status. Mutational analysis has identified a single conserved cysteine residue mapping within the DNA binding domains of Fos and Jun. Chemical oxidation or modification of this cysteine residue inhibits the DNA binding activity of Fos and Jun. A similar mode of regulation has been recently proposed for other nuclear transcription factors. Oxidation is reversible by these compounds or by a cellular redox/DNA repair protein identified originally as Ref-1 (redox factor 1). Ref-1 is identical to a previously characterized DNA repair enzyme designated HAP1, APE or APEX.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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