Anti-Ref-1 Antibody (C-4) recommended for detection of Ref-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Anti-Ref-1 Antibody (C-4): sc-17774

Anti-Ref-1 Antibody (C-4) is rated 4.7 out of 5 by 20.
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Datasheets

    • Anti-Ref-1 Antibody (C-4) is a mouse monoclonal IgG2b κ Ref-1 antibody, cited in 49 publications, provided at 200 µg/ml
    • raised against amino acids 1-300 of Ref-1 of human origin
    • Anti-Ref-1 Antibody (C-4) is recommended for detection of Ref-1 of mouse, rat and human origin by WB, IP, IF and IHC(P)
    • Anti-Ref-1 Antibody (C-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-17774 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Ref-1 (C-4): sc-17774.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for Ref-1 Antibody (C-4) for WB and IHC(P) applications. These reagents are now offered in bundles with Ref-1 Antibody (C-4) (see ordering information below).

Does Ref-1 Antibody (C-4): sc-17774 react with Avian samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 13
Date published: 2017-01-20

I am looking to use Ref-1 Antibody (C-4): sc-17774 for both ChIP and Western blot applications. Should I purchase the “X” form?

Asked by: Trav11
Thank you for your question. We typically recommend purchasing the “X” form only if you are going to primarily be using the antibody for ChIP applications. But, as you are going to be using the antibody for both ChIP and WB, I would recommend purchasing the standard Ref-1 Antibody (C-4): sc-17774. If you have any further questions or concerns, please feel free to contact the Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Service 11
Date published: 2017-01-16
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Rated 5 out of 5 by from Good signal Good, clean signal in WB of total lysates of human cell lines.
Date published: 2020-10-27
Rated 4 out of 5 by from Buon segnale pulito La colorazione è buona, per avere un segnale un po' più forte è preferibile fare un'incubazione overnight dell'anticorpo, anche a scapito di una leggera colorazione aspecifica nel citoplasma di alcune cellule
Date published: 2020-06-01
Rated 5 out of 5 by from great antibody for WB Antibody is very easy to use, 1: 1000 ratio dilution, the band is very good
Date published: 2017-09-26
Rated 3 out of 5 by from review Maybe I do not find a suit condition.Anyway ,the result is not what I want.
Date published: 2017-09-20
Rated 5 out of 5 by from Great antibody Antibody works well - clear, specific band for western blot with 25 ug U87 cell lysates at 1:1000 primary antibody dilution.
Date published: 2017-09-10
Rated 5 out of 5 by from Great Product This Ab is superb every time I used it given me best result. The min benefit of it is it not too concentrated and provide accurate result even in 1:1000 or in 1:2000 dilution also.
Date published: 2017-09-05
Rated 5 out of 5 by from immunostaining The results showed that immunostaining was clear by dilluted 1/150. And the WB had a signal band.
Date published: 2017-05-04
Rated 5 out of 5 by from Wow !! Outstanding Superb WB,IF and IHC data; much better product than any of the discontinued polyclonals
Date published: 2017-01-24
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