Raftlin Antibody (E-11): sc-514457
- Raftlin Antibody (E-11) is a mouse monoclonal IgG2b κ Raftlin antibody provided at 200 µg/ml
- raised against amino acids 29-328 mapping within an internal region of Raftlin of mouse origin
- Raftlin Antibody (E-11) is recommended for detection of Raftlin of mouse origin by WB, IP, IF and ELISA
- Anti-Raftlin Antibody (E-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Raftlin (E-11): sc-514457.
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Raftlin Antibody (E-11) for WB applications. These reagents are now offered in bundles with Raftlin Antibody (E-11) (see ordering information below).
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Raftlin Antibody (E-11) is a mouse monoclonal IgG2b kappa light chain antibody that detects Raftlin protein of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Raftlin (E-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Raftlin, also known as RFTN1 (raftlin, lipid raft linker 1), plays a crucial role in organizing and functioning of lipid rafts, which are specialized membrane microdomains that facilitate B-cell activation during B-cell receptor signaling. Raftlin (E-11) monoclonal antibody targets a protein primarily located in the cell membrane, where Raftlin acts as a lipid anchor, essential for the assembly and maintenance of these rafts. The proper functioning of lipid rafts is vital for effective immune responses, as these specialized structures serve as platforms for clustering signaling molecules, thereby enhancing signal transduction pathway efficiency. The gene encoding Raftlin is situated on human chromosome 3, a region associated with various genetic disorders and cancer-related genes, underscoring Raftlin′s importance in both normal cellular function and disease processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Raftlin Antibody (E-11) References:
- Mapping of the CCXCR1, CX3CR1, CCBP2 and CCR9 genes to the CCR cluster within the 3p21.3 region of the human genome. | Maho, A., et al. 1999. Cytogenet Cell Genet. 87: 265-8. PMID: 10702689
- [New tumor suppressor genes in hot spots of human chromosome 3: new methods of identification]. | Braga, EA., et al. 2003. Mol Biol (Mosk). 37: 194-211. PMID: 12723467
- The B cell-specific major raft protein, Raftlin, is necessary for the integrity of lipid raft and BCR signal transduction. | Saeki, K., et al. 2003. EMBO J. 22: 3015-26. PMID: 12805216
- Methylation of the tumor suppressor gene RASSF1A in human tumors. | Pfeifer, GP. and Dammann, R. 2005. Biochemistry (Mosc). 70: 576-83. PMID: 15948711
- A major lipid raft protein raftlin modulates T cell receptor signaling and enhances th17-mediated autoimmune responses. | Saeki, K., et al. 2009. J Immunol. 182: 5929-37. PMID: 19414744
- Uptake of genetic testing and long-term tumor surveillance in von Hippel-Lindau disease. | Rasmussen, A., et al. 2010. BMC Med Genet. 11: 4. PMID: 20064270
- Mutilating neuropathic ulcerations in a chromosome 3q13-q22 linked Charcot-Marie-Tooth disease type 2B family. | De Jonghe, P., et al. 1997. J Neurol Neurosurg Psychiatry. 62: 570-3. PMID: 9219740
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Raftlin Antibody (E-11) | sc-514457 | 200 µg/ml | $316.00 | |||
Raftlin Antibody (E-11): m-IgG Fc BP-HRP Bundle | sc-531134 | 200 µg Ab; 10 µg BP | $354.00 | |||
Raftlin Antibody (E-11): m-IgGκ BP-HRP Bundle | sc-524734 | 200 µg Ab, 40 µg BP | $354.00 | |||
Raftlin Antibody (E-11): m-IgG2b BP-HRP Bundle | sc-550259 | 200 µg Ab; 10 µg BP | $354.00 | |||
Raftlin Antibody (E-11) AC | sc-514457 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Raftlin Antibody (E-11) HRP | sc-514457 HRP | 200 µg/ml | $316.00 | |||
Raftlin Antibody (E-11) FITC | sc-514457 FITC | 200 µg/ml | $330.00 | |||
Raftlin Antibody (E-11) PE | sc-514457 PE | 200 µg/ml | $343.00 | |||
Raftlin Antibody (E-11) Alexa Fluor® 488 | sc-514457 AF488 | 200 µg/ml | $357.00 | |||
Raftlin Antibody (E-11) Alexa Fluor® 546 | sc-514457 AF546 | 200 µg/ml | $357.00 | |||
Raftlin Antibody (E-11) Alexa Fluor® 594 | sc-514457 AF594 | 200 µg/ml | $357.00 | |||
Raftlin Antibody (E-11) Alexa Fluor® 647 | sc-514457 AF647 | 200 µg/ml | $357.00 | |||
Raftlin Antibody (E-11) Alexa Fluor® 680 | sc-514457 AF680 | 200 µg/ml | $357.00 | |||
Raftlin Antibody (E-11) Alexa Fluor® 790 | sc-514457 AF790 | 200 µg/ml | $357.00 |