Polycystin-2 Antibody (D-3): sc-28331
- Polycystin-2 Antibody (D-3) is a mouse monoclonal IgG2b κ, cited in 33 publications, provided at 200 µg/ml
- raised against amino acids 689-968 of Polycystin-2 of human origin
- Anti-Polycystin-2 Antibody (D-3) is recommended for detection of Polycystin-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Polycystin-2 Antibody (D-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Polycystin-2 Antibody (D-3) for WB and IHC(P) applications. These reagents are now offered in bundles with Polycystin-2 Antibody (D-3) (see ordering information below).
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Polycystin-2 Antibody (D-3) is a mouse monoclonal IgG2b kappa light chain antibody that specifically targets Polycystin-2, also known as PKD2, with reactivity confirmed across human, rat, and mouse origins in applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Polycystin-2 is a crucial transmembrane protein involved in calcium signaling and is integral to maintaining normal kidney function, as PKD2 regulates ion transport within cells. Defects in Polycystin-2 are implicated in autosomal dominant polycystic kidney disease (ADPKD), where mutations contribute to the formation of cysts within kidney tubules, leading to progressive kidney dysfunction. PKD2 localizes primarily within kidney structures like medullary and cortical collecting ducts and distal convoluted tubules and interacts with Polycystin-1 to regulate AP-1 signaling, impacting cell proliferation, differentiation, and apoptosis. Through association with Hax-1, Polycystin-2 may also influence cell-matrix interactions, further supporting cellular stability and function. Polycystin-2 (D-3) antibody is available as a non-conjugated format or with multiple conjugates, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® options, allowing versatile application in detecting and studying PKD2′s role in ADPKD and other cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Polycystin-2 Antibody (D-3) References:
- Cellular activation triggered by the autosomal dominant polycystic kidney disease gene product PKD2. | Arnould, T., et al. 1999. Mol Cell Biol. 19: 3423-34. PMID: 10207066
- Coordinate expression of the autosomal dominant polycystic kidney disease proteins, polycystin-2 and polycystin-1, in normal and cystic tissue. | Ong, AC., et al. 1999. Am J Pathol. 154: 1721-9. PMID: 10362797
- Identification and characterization of polycystin-2, the PKD2 gene product. | Cai, Y., et al. 1999. J Biol Chem. 274: 28557-65. PMID: 10497221
- Polycystin-1, the PKD1 gene product, is in a complex containing E-cadherin and the catenins. | Huan, Y. and van Adelsberg, J. 1999. J Clin Invest. 104: 1459-68. PMID: 10562308
- The rat pkd2 protein assumes distinct subcellular distributions in different organs. | Obermüller, N., et al. 1999. Am J Physiol. 277: F914-25. PMID: 10600939
- Cardiac defects and renal failure in mice with targeted mutations in Pkd2. | Wu, G., et al. 2000. Nat Genet. 24: 75-8. PMID: 10615132
- The polycystic kidney disease protein PKD2 interacts with Hax-1, a protein associated with the actin cytoskeleton. | Gallagher, AR., et al. 2000. Proc Natl Acad Sci U S A. 97: 4017-22. PMID: 10760273
- The polycystic kidney disease 1 gene product mediates protein kinase C alpha-dependent and c-Jun N-terminal kinase-dependent activation of the transcription factor AP-1. | Arnould, T., et al. 1998. J Biol Chem. 273: 6013-8. PMID: 9497315
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Polycystin-2 Antibody (D-3) | sc-28331 | 200 µg/ml | $322.00 | |||
Polycystin-2 Antibody (D-3): m-IgG Fc BP-HRP Bundle | sc-528427 | 200 µg Ab; 10 µg BP | $361.00 | |||
Polycystin-2 Antibody (D-3): m-IgGκ BP-HRP Bundle | sc-520801 | 200 µg Ab, 40 µg BP | $361.00 | |||
Polycystin-2 Antibody (D-3): m-IgG2b BP-HRP Bundle | sc-550036 | 200 µg Ab; 10 µg BP | $361.00 | |||
Polycystin-2 Antibody (D-3) AC | sc-28331 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Polycystin-2 Antibody (D-3) HRP | sc-28331 HRP | 200 µg/ml | $322.00 | |||
Polycystin-2 Antibody (D-3) FITC | sc-28331 FITC | 200 µg/ml | $336.00 | |||
Polycystin-2 Antibody (D-3) PE | sc-28331 PE | 200 µg/ml | $349.00 | |||
Polycystin-2 Antibody (D-3) Alexa Fluor® 488 | sc-28331 AF488 | 200 µg/ml | $364.00 | |||
Polycystin-2 Antibody (D-3) Alexa Fluor® 546 | sc-28331 AF546 | 200 µg/ml | $364.00 | |||
Polycystin-2 Antibody (D-3) Alexa Fluor® 594 | sc-28331 AF594 | 200 µg/ml | $364.00 | |||
Polycystin-2 Antibody (D-3) Alexa Fluor® 647 | sc-28331 AF647 | 200 µg/ml | $364.00 | |||
Polycystin-2 Antibody (D-3) Alexa Fluor® 680 | sc-28331 AF680 | 200 µg/ml | $364.00 | |||
Polycystin-2 Antibody (D-3) Alexa Fluor® 790 | sc-28331 AF790 | 200 µg/ml | $364.00 |