POLR2J1/2/3 Antibody (G-2): sc-514129

POLR2J1/2/3 Antibody (G-2) is a mouse monoclonal IgG1 antibody that detects POLR2J1, POLR2J2, and POLR2J3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). POLR2J1/2/3 (G-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. POLR2J proteins, which are subunits of RNA polymerase II, play a crucial role in the transcription of protein-coding genes, a process essential for gene expression and cellular function. These proteins are located in the nucleus, where they form part of the transcription machinery that interacts with promoter regions of genes, facilitating the recruitment of transcription factors and other regulatory elements. Proper functioning of POLR2J proteins is vital, as any disruption in their activity can lead to impaired transcription and has been associated with various diseases, including cancer. Understanding the dynamics of POLR2J1, POLR2J2, and POLR2J3 is important for elucidating the mechanisms of gene regulation and potential therapeutic targets for transcription-related disorders.

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POLR2J1/2/3 Antibody (G-2) References:

1. Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms.  |  Bushnell, DA., et al. 2004. Science. 303: 983-8. PMID: 14963322
2. BRCA1 cooperates with NUFIP and P-TEFb to activate transcription by RNA polymerase II.  |  Cabart, P., et al. 2004. Oncogene. 23: 5316-29. PMID: 15107825
3. Epidermal growth factor enhances cellular TATA binding protein levels and induces RNA polymerase I- and III-dependent gene activity.  |  Zhong, S., et al. 2004. Mol Cell Biol. 24: 5119-29. PMID: 15169879
4. Downstream DNA selectively affects a paused conformation of human RNA polymerase II.  |  Palangat, M., et al. 2004. J Mol Biol. 341: 429-42. PMID: 15276834
5. [New genes on human chromosome 7: bioinformatic analysis of a gene cluster from the POLR2J family].  |  Shpakovskiĭ, DG., et al. 2004. Bioorg Khim. 30: 621-5. PMID: 15586814
6. Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II.  |  Meka, H., et al. 2005. Nucleic Acids Res. 33: 6435-44. PMID: 16282592
7. RNA emerging from the active site of RNA polymerase II interacts with the Rpb7 subunit.  |  Ujvári, A. and Luse, DS. 2006. Nat Struct Mol Biol. 13: 49-54. PMID: 16327806
8. Genomic location of the human RNA polymerase II general machinery: evidence for a role of TFIIF and Rpb7 at both early and late stages of transcription.  |  Cojocaru, M., et al. 2008. Biochem J. 409: 139-47. PMID: 17848138

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-POLR2J1/2/3 Antibody (G-2) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-POLR2J1/2/3 Antibody (G-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

POLR2J1/2/3 Antibody (G-2) Specifications

Alternate Product Name: POLR2J1/2/3

Product Clone Name: G-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 41-117

Epitope Target: POLR2J1

Epitope Species: human

Product Reactivity/Detection: POLR2J1, POLR2J2 and POLR2J3 of human origin, and Polr2j of mouse and rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for POLR2J1/2/3 Antibody (G-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent