POLR2I Antibody (F-11): sc-398049
- POLR2I Antibody (F-11) is a mouse monoclonal IgG1 κ POLR2I antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 1-120 mapping at the N-terminus of POLR2I of human origin
- POLR2I Antibody (F-11) is recommended for detection of POLR2I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-POLR2I Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for POLR2I Antibody (F-11) for WB and IHC(P) applications. These reagents are now offered in bundles with POLR2I Antibody (F-11) (see ordering information below).
POLR2I Antibody (F-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects POLR2I protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-POLR2I antibody (F-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. POLR2I protein, also known as polymerase (RNA) II (DNA directed) polypeptide I, RPB9, or hRPB14.5, is a crucial component of RNA polymerase II, which is responsible for the transcription of protein-coding genes. POLR2I consists of 125 amino acids and belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. POLR2I plays a vital role in the transcription process by facilitating the recruitment of the transcription machinery to gene promoters, which is essential for the initiation of transcription. POLR2I′s location within the upper jaw of the RNA polymerase II complex allows interaction with the incoming DNA template, ensuring accurate transcription of genetic information into RNA. POLR2I′s coordination with other subunits and transcription factors highlights its importance in gene expression regulation, making POLR2I a key target for studies related to transcriptional control and its implications in various biological processes and diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
POLR2I Antibody (F-11) References:
- Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms. | Bushnell, DA., et al. 2004. Science. 303: 983-8. PMID: 14963322
- BRCA1 cooperates with NUFIP and P-TEFb to activate transcription by RNA polymerase II. | Cabart, P., et al. 2004. Oncogene. 23: 5316-29. PMID: 15107825
- Epidermal growth factor enhances cellular TATA binding protein levels and induces RNA polymerase I- and III-dependent gene activity. | Zhong, S., et al. 2004. Mol Cell Biol. 24: 5119-29. PMID: 15169879
- Distinct mechanisms for repression of RNA polymerase III transcription by the retinoblastoma tumor suppressor protein. | Hirsch, HA., et al. 2004. Mol Cell Biol. 24: 5989-99. PMID: 15199152
- Downstream DNA selectively affects a paused conformation of human RNA polymerase II. | Palangat, M., et al. 2004. J Mol Biol. 341: 429-42. PMID: 15276834
- Chromosomal localization of human RNA polymerase II subunit genes. | Acker, J., et al. 1994. Genomics. 20: 496-9. PMID: 8034326
- Structure of the gene encoding the 14.5 kDa subunit of human RNA polymerase II. | Acker, J., et al. 1993. Nucleic Acids Res. 21: 5345-50. PMID: 8265347
- Six human RNA polymerase subunits functionally substitute for their yeast counterparts. | McKune, K., et al. 1995. Mol Cell Biol. 15: 6895-900. PMID: 8524256
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
POLR2I Antibody (F-11) | sc-398049 | 200 µg/ml | $322.00 | |||
POLR2I Antibody (F-11): m-IgG Fc BP-HRP Bundle | sc-530679 | 200 µg Ab; 10 µg BP | $361.00 | |||
POLR2I Antibody (F-11): m-IgGκ BP-HRP Bundle | sc-524199 | 200 µg Ab, 40 µg BP | $361.00 | |||
POLR2I Antibody (F-11): m-IgG1 BP-HRP Bundle | sc-544195 | 200 µg Ab; 20 µg BP | $361.00 | |||
POLR2I Antibody (F-11) AC | sc-398049 AC | 500 µg/ml, 25% agarose | $424.00 | |||
POLR2I Antibody (F-11) HRP | sc-398049 HRP | 200 µg/ml | $322.00 | |||
POLR2I Antibody (F-11) FITC | sc-398049 FITC | 200 µg/ml | $336.00 | |||
POLR2I Antibody (F-11) PE | sc-398049 PE | 200 µg/ml | $349.00 | |||
POLR2I Antibody (F-11) Alexa Fluor® 488 | sc-398049 AF488 | 200 µg/ml | $364.00 | |||
POLR2I Antibody (F-11) Alexa Fluor® 546 | sc-398049 AF546 | 200 µg/ml | $364.00 | |||
POLR2I Antibody (F-11) Alexa Fluor® 594 | sc-398049 AF594 | 200 µg/ml | $364.00 | |||
POLR2I Antibody (F-11) Alexa Fluor® 647 | sc-398049 AF647 | 200 µg/ml | $364.00 | |||
POLR2I Antibody (F-11) Alexa Fluor® 680 | sc-398049 AF680 | 200 µg/ml | $364.00 | |||
POLR2I Antibody (F-11) Alexa Fluor® 790 | sc-398049 AF790 | 200 µg/ml | $364.00 |