POLR1E Antibody (C-11): sc-398270

POLR1E Antibody (C-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects POLR1E protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). POLR1E (C-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. POLR1E protein, also known as polymerase (RNA) I polypeptide E, PAF53, or PRAF1, is a crucial component of the RNA polymerase I complex, which synthesizes ribosomal RNA precursors essential for ribosome biogenesis. This 481 amino acid protein is primarily localized in the nucleolus, where POLR1E plays a vital role in transcribing DNA into RNA, utilizing ribonucleoside triphosphates as substrates. Proper functioning of POLR1E is critical for maintaining cellular homeostasis and supporting protein synthesis, as ribosomal RNA is a fundamental component of ribosomes, the cellular machinery responsible for translating messenger RNA into proteins. The gene encoding POLR1E is located on human chromosome 9, a region associated with various genetic disorders, including hereditary hemorrhagic telangiectasia and familial dysautonomia, highlighting POLR1E′s importance in both normal physiology and disease states.

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POLR1E Antibody (C-11) References:

1. Phosphorylation of UBF at serine 388 is required for interaction with RNA polymerase I and activation of rDNA transcription.  |  Voit, R. and Grummt, I. 2001. Proc Natl Acad Sci U S A. 98: 13631-6. PMID: 11698641
2. Regulation of ribosomal RNA synthesis during the final phases of porcine oocyte growth.  |  Bjerregaard, B., et al. 2004. Biol Reprod. 70: 925-35. PMID: 14627545
3. Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription.  |  Yamamoto, K., et al. 2004. Mol Cell Biol. 24: 6338-49. PMID: 15226435
4. The chromatin remodelling complex WSTF-SNF2h interacts with nuclear myosin 1 and has a role in RNA polymerase I transcription.  |  Percipalle, P., et al. 2006. EMBO Rep. 7: 525-30. PMID: 16514417
5. RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor.  |  Panov, KI., et al. 2006. Mol Cell Biol. 26: 5436-48. PMID: 16809778
6. AC/D(eA)C rocks the polymerases.  |  Laitem, C. and Murphy, S. 2013. Mol Cell. 52: 287-8. PMID: 24207021
7. Heterozygous De Novo UBTF Gain-of-Function Variant Is Associated with Neurodegeneration in Childhood.  |  Edvardson, S., et al. 2017. Am J Hum Genet. 101: 267-273. PMID: 28777933
8. Polymerase-1 pathway activation in acute multiple sclerosis relapse.  |  Achiron, A., et al. 2018. Autoimmun Rev. 17: 1235-1239. PMID: 30316990
9. Discovery of novel predisposing coding and noncoding variants in familial Hodgkin lymphoma.  |  Flerlage, JE., et al. 2023. Blood. 141: 1293-1307. PMID: 35977101
10. Screening seven hub genes associated with prognosis and immune infiltration in glioblastoma.  |  Zhang, Y., et al. 2022. Front Genet. 13: 924802. PMID: 36035134
11. PAF49: An RNA Polymerase I subunit essential for rDNA transcription and stabilization of PAF53.  |  McNamar, R., et al. 2023. J Biol Chem. 299: 104951. PMID: 37356716
12. Constitutive and strong association of PAF53 with RNA polymerase I.  |  Seither, P., et al. 1997. Chromosoma. 106: 216-25. PMID: 9254723

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-POLR1E Antibody (C-11) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-POLR1E Antibody (C-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

POLR1E Antibody (C-11) Specifications

Alternate Product Name: POLR1E

Product Clone Name: C-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 8-113

Epitope Target: POLR1E

Epitope Species: human

Product Reactivity/Detection: POLR1E

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for POLR1E Antibody (C-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent