Pol II Antibody (A-10): sc-17798
- Pol II Antibody (A-10) is a mouse monoclonal IgG2b κ, cited in 48 publications, provided at 200 µg/ml
- raised against amino acids 1-224 of Pol II of human origin
- Anti-Pol II Antibody (A-10) is recommended for detection of Pol II of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Pol II Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Pol II Antibody (A-10) for WB applications. These reagents are now offered in bundles with Pol II Antibody (A-10) (see ordering information below).
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SEE ALSO...
Pol II Antibody (A-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects RNA Polymerase II (Pol II) in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Pol II antibody (A-10) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RNA Polymerase II plays a crucial role in transcribing protein-coding genes, and proper function is essential for gene expression regulation. RNA Polymerase II consists of twelve subunits, with RPB1 (also known as RPB205) being a 1,840 amino acid protein featuring a C2H2-type zinc finger and a C-terminal domain rich in heptapeptide repeats. RPB1 is vital for catalytic activity and forming the DNA-binding domain necessary for DNA template transcription. RPB1′s interaction with the second largest subunit is critical for forming the active center of RNA Polymerase II, and dysfunction can lead to impaired mRNA synthesis and transcription elongation. Pol II (A-10) monoclonal antibody serves as an invaluable tool for researchers studying transcriptional regulation and molecular mechanisms underlying gene expression in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pol II Antibody (A-10) | sc-17798 | 200 µg/ml | $322.00 | |||
Pol II Antibody (A-10): m-IgGκ BP-HRP Bundle | sc-520662 | 200 µg Ab, 40 µg BP | $361.00 | |||
Pol II Antibody (A-10): m-IgG2b BP-HRP Bundle | sc-548845 | 200 µg Ab; 10 µg BP | $361.00 | |||
Pol II Antibody (A-10) AC | sc-17798 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Pol II Antibody (A-10) HRP | sc-17798 HRP | 200 µg/ml | $322.00 | |||
Pol II Antibody (A-10) FITC | sc-17798 FITC | 200 µg/ml | $336.00 | |||
Pol II Antibody (A-10) PE | sc-17798 PE | 200 µg/ml | $349.00 | |||
Pol II Antibody (A-10) Alexa Fluor® 488 | sc-17798 AF488 | 200 µg/ml | $364.00 | |||
Pol II Antibody (A-10) Alexa Fluor® 546 | sc-17798 AF546 | 200 µg/ml | $364.00 | |||
Pol II Antibody (A-10) Alexa Fluor® 594 | sc-17798 AF594 | 200 µg/ml | $364.00 | |||
Pol II Antibody (A-10) Alexa Fluor® 647 | sc-17798 AF647 | 200 µg/ml | $364.00 | |||
Pol II Antibody (A-10) Alexa Fluor® 680 | sc-17798 AF680 | 200 µg/ml | $364.00 | |||
Pol II Antibody (A-10) Alexa Fluor® 790 | sc-17798 AF790 | 200 µg/ml | $364.00 |