Pol II Antibody (8WG16): sc-56767
- Pol II Antibody (8WG16) is a mouse monoclonal IgG2a κ, cited in 104 publications, provided at 200 µg/ml
- raised against purified RNA pol II of wheat germ origin
- Anti-Pol II Antibody (8WG16) is recommended for detection of the highly conserved heptapeptide repeat of the largest subunit of eukaryotic Pol II of mouse, rat, human, Xenopus, C. elegans, yeast, wheat germ and bovine origin by WB, IP and IF
- Anti-Pol II Antibody (8WG16) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Pol II (8WG16): sc-56767.
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Pol II Antibody (8WG16) for WB applications. These reagents are now offered in bundles with Pol II Antibody (8WG16) (see ordering information below).
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SEE ALSO...
Pol II Antibody (8WG16) is a mouse monoclonal IgG2a kappa light chain antibody that detects RNA Polymerase II (Pol II) across various species including mouse, rat, human, Xenopus, C. elegans, yeast, wheat germ, and bovine through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). RNA Polymerase II (Pol II) antibody (8WG16) is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates. RNA Polymerase II is a crucial enzyme composed of twelve subunits, responsible for the transcription of protein-coding genes, which is essential for gene expression and cellular function. The largest subunit of RNA Polymerase II, known as RPB1 or RPB205, is a 1,840 amino acid protein that features a C2H2-type zinc finger and a C-terminal domain rich in heptapeptide repeats. This structural composition is vital as RNA Polymerase II not only facilitates catalytic activity but also plays a key role in forming the DNA-binding domain, which is necessary for the accurate transcription of the DNA template. The integrity of the largest subunit is critical; without proper function, the processes of mRNA synthesis and transcription elongation are severely compromised, highlighting RNA Polymerase II′s importance in maintaining cellular homeostasis and regulating gene expression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Pol II Antibody (8WG16) References:
- Structural basis of transcription: an RNA polymerase II-TFIIB cocrystal at 4.5 Angstroms. | Bushnell, DA., et al. 2004. Science. 303: 983-8. PMID: 14963322
- Structure and function of RNA polymerase II. | Cramer, P. 2004. Adv Protein Chem. 67: 1-42. PMID: 14969722
- Mechanism of RNA polymerase I transcription. | Comai, L. 2004. Adv Protein Chem. 67: 123-55. PMID: 14969726
- The RNA polymerase II transcription cycle: cycling through chromatin. | Svejstrup, JQ. 2004. Biochim Biophys Acta. 1677: 64-73. PMID: 15020047
- RNA polymerase III transcription and cancer. | White, RJ. 2004. Oncogene. 23: 3208-16. PMID: 15094770
- BRCA1 cooperates with NUFIP and P-TEFb to activate transcription by RNA polymerase II. | Cabart, P., et al. 2004. Oncogene. 23: 5316-29. PMID: 15107825
- Epidermal growth factor enhances cellular TATA binding protein levels and induces RNA polymerase I- and III-dependent gene activity. | Zhong, S., et al. 2004. Mol Cell Biol. 24: 5119-29. PMID: 15169879
- Distinct mechanisms for repression of RNA polymerase III transcription by the retinoblastoma tumor suppressor protein. | Hirsch, HA., et al. 2004. Mol Cell Biol. 24: 5989-99. PMID: 15199152
- Downstream DNA selectively affects a paused conformation of human RNA polymerase II. | Palangat, M., et al. 2004. J Mol Biol. 341: 429-42. PMID: 15276834
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pol II Antibody (8WG16) | sc-56767 | 200 µg/ml | $333.00 | |||
Pol II Antibody (8WG16): m-IgG Fc BP-HRP Bundle | sc-537117 | 200 µg Ab; 10 µg BP | $354.00 | |||
Pol II Antibody (8WG16): m-IgGκ BP-HRP Bundle | sc-534309 | 200 µg Ab; 40 µg BP | $354.00 | |||
Pol II Antibody (8WG16): m-IgG2a BP-HRP Bundle | sc-547929 | 200 µg Ab; 10 µg BP | $354.00 | |||
Pol II Antibody (8WG16) AC | sc-56767 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Pol II Antibody (8WG16) HRP | sc-56767 HRP | 200 µg/ml | $316.00 | |||
Pol II Antibody (8WG16) FITC | sc-56767 FITC | 200 µg/ml | $330.00 | |||
Pol II Antibody (8WG16) PE | sc-56767 PE | 200 µg/ml | $343.00 | |||
Pol II Antibody (8WG16) Alexa Fluor® 488 | sc-56767 AF488 | 200 µg/ml | $357.00 | |||
Pol II Antibody (8WG16) Alexa Fluor® 546 | sc-56767 AF546 | 200 µg/ml | $357.00 | |||
Pol II Antibody (8WG16) Alexa Fluor® 594 | sc-56767 AF594 | 200 µg/ml | $357.00 | |||
Pol II Antibody (8WG16) Alexa Fluor® 647 | sc-56767 AF647 | 200 µg/ml | $357.00 | |||
Pol II Antibody (8WG16) Alexa Fluor® 680 | sc-56767 AF680 | 200 µg/ml | $357.00 | |||
Pol II Antibody (8WG16) Alexa Fluor® 790 | sc-56767 AF790 | 200 µg/ml | $357.00 |