PME-1 Antibody (A-10): sc-137145

PME-1 Antibody (A-10) is a mouse monoclonal IgG2b antibody that detects protein phosphatase methylesterase-1 (PME-1) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PME-1 plays a crucial role in regulating protein phosphatase 2A (PP2A) activity, a key enzyme involved in cell growth inhibition and cell cycle regulation. By catalyzing PP2A demethylation and inactivation, PME-1 influences various cellular processes, including proliferation and apoptosis, making PME-1 essential for maintaining cellular homeostasis. PME-1 is primarily located in the cytoplasm, where PME-1 interacts with PP2A and other regulatory proteins, underscoring PME-1′s importance in modulating signaling pathways that govern cell fate decisions. PME-1 is highly conserved across species, from yeast to humans, and features a catalytic triad similar to that found in lipases, which is critical for enzymatic function. PME-1′s ability to demethylate PP2A catalytic subunit in vitro, along with reaction inhibition by okadaic acid, highlights PME-1′s potential as a therapeutic target in diseases characterized by dysregulated phosphatase activity. Anti-PME-1 antibody (A-10) is an invaluable tool for researchers studying cell signaling mechanisms and phosphatase roles in various biological contexts.

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PME-1 Antibody (A-10) References:

1. Academic cross-fertilization by public screening yields a remarkable class of protein phosphatase methylesterase-1 inhibitors.  |  Bachovchin, DA., et al. 2011. Proc Natl Acad Sci U S A. 108: 6811-6. PMID: 21398589
2. Discovery and optimization of sulfonyl acrylonitriles as selective, covalent inhibitors of protein phosphatase methylesterase-1.  |  Bachovchin, DA., et al. 2011. J Med Chem. 54: 5229-36. PMID: 21639134
3. Glycogen synthase kinase-3β regulates leucine-309 demethylation of protein phosphatase-2A via PPMT1 and PME-1.  |  Yao, XQ., et al. 2012. FEBS Lett. 586: 2522-8. PMID: 22732552
4. A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size.  |  Xia, X., et al. 2015. Cell Cycle. 14: 1938-47. PMID: 25839665
5. PP2A Inhibitor PME-1 Drives Kinase Inhibitor Resistance in Glioma Cells.  |  Kaur, A., et al. 2016. Cancer Res. 76: 7001-7011. PMID: 27671680
6. PME-1 is regulated by USP36 in ERK and Akt signaling pathways.  |  Kim, SY., et al. 2018. FEBS Lett. 592: 1575-1588. PMID: 29577269
7. Expression Pattern and Prognostic Utility of PME-1 in Patients with Hepatocellular Carcinoma.  |  Du, B., et al. 2020. Cancer Manag Res. 12: 2937-2945. PMID: 32431540
8. Coupling to short linear motifs creates versatile PME-1 activities in PP2A holoenzyme demethylation and inhibition.  |  Li, Y., et al. 2022. Elife. 11: PMID: 35924897
9. PME-1 sensitizes glioblastoma cells to oxidative stress-induced cell death by attenuating PP2A-B55α-mediated inactivation of MAPKAPK2-RIPK1 signaling.  |  Guffens, L., et al. 2023. Cell Death Discov. 9: 265. PMID: 37500619
10. Transcriptome analysis revealed that PME-1 suppresses inflammatory signaling, activates PI3K/Akt signaling, and promotes epithelial-mesenchymal transition.  |  Ikeda, S., et al. 2024. Biochem Biophys Res Commun. 692: 149148. PMID: 38043157

The preferred PME-1 antibody is PME-1 Antibody (B-12): sc-25278.