PLC β1 Antibody (D-8): sc-5291

PLC β1 Antibody (D-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects the PLC beta 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PLC β1 (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The phosphoinositide-specific phospholipase C (PLC) family, which includes PLC β1, is essential for initiating receptor-mediated signal transduction by generating the second messengers inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This process is critical for various cellular functions, including cell proliferation, differentiation, and response to growth factors. PLC β1, along with its isoforms, is involved in diverse physiological processes and is particularly important in the regulation of calcium signaling pathways. PLC β1′s ability to interact with other signaling molecules and role in modulating cellular responses highlight its significance in maintaining cellular homeostasis and mediating responses to external stimuli. Understanding PLC β1 function and interactions can provide insights into potential implications in various diseases, including cancer and cardiovascular disorders, making PLC β1 a valuable target for research and therapeutic applications.

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PLC β1 Antibody (D-8) References:

1. PLC-beta1, activated via mGluRs, mediates activity-dependent differentiation in cerebral cortex.  |  Hannan, AJ., et al. 2001. Nat Neurosci. 4: 282-8. PMID: 11224545
2. Regulation of inositol phospholipid-specific phospholipase C isozymes.  |  Rhee, SG. and Choi, KD. 1992. J Biol Chem. 267: 12393-6. PMID: 1319994
3. A second gene product of the inositol-phospholipid-specific phospholipase C delta subclass.  |  Meldrum, E., et al. 1991. Eur J Biochem. 196: 159-65. PMID: 1848183
4. A second type of rat phosphoinositide-specific phospholipase C containing a src-related sequence not essential for phosphoinositide-hydrolyzing activity.  |  Emori, Y., et al. 1989. J Biol Chem. 264: 21885-90. PMID: 2557343
5. Modulation of nuclear PI-PLCbeta1 during cell differentiation.  |  Cocco, L., et al. 2016. Adv Biol Regul. 60: 1-5. PMID: 26525203
6. Inositol phospholipid-specific phospholipase C: complete cDNA and protein sequences and sequence homology to tyrosine kinase-related oncogene products.  |  Suh, PG., et al. 1988. Proc Natl Acad Sci U S A. 85: 5419-23. PMID: 2840660
7. Cell-autonomous PLCβ1 modulation of neural stem/progenitor cell proliferation during adult hippocampal neurogenesis.  |  Song, B. and Kim, CH. 2022. Neurosci Lett. 791: 136899. PMID: 36202196
8. Cloning of cDNA encoding rat phospholipase C-beta 4, a new member of the phospholipase C.  |  Kim, MJ., et al. 1993. Biochem Biophys Res Commun. 194: 706-12. PMID: 7688223
9. Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3.  |  Jhon, DY., et al. 1993. J Biol Chem. 268: 6654-61. PMID: 8454637
10. Carboxyl-terminal fragments of phospholipase C-beta1 with intrinsic Gq GTPase-activating protein (GAP) activity.  |  Paulssen, RH., et al. 1996. J Biol Chem. 271: 26622-9. PMID: 8900136
11. Phospholipase C-beta1 is present in the botrysome, an intermediate compartment-like organelle, and Is regulated by visual experience in cat visual cortex.  |  Kind, PC., et al. 1997. J Neurosci. 17: 1471-80. PMID: 9006988
12. Localization of two forms of phospholipase C-beta1, a and b, in C6Bu-1 cells.  |  Bahk, YY., et al. 1998. Biochim Biophys Acta. 1389: 76-80. PMID: 9443606

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PLC β1 Antibody (D-8) has 46 citations in a variety of scientific publications.

Primary antibodies like Anti-PLC β1 Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PLC β1 Antibody (D-8) Specifications

Alternate Product Name: PLCB1

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 831-1063

Epitope Target: PLC β1

Epitope Species: rat

Product Reactivity/Detection: PLC β1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 46 citations in various scientific publications

Available Conjugates/Forms for PLC β1 Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin