PERK Antibody (B-5): sc-377400
- PERK Antibody (B-5) is a mouse monoclonal IgG1 κ PERK antibody, cited in 112 publications, provided at 200 µg/ml
- raised against amino acids 21-320 mapping near the N-terminus of PERK of human origin
- PERK Antibody (B-5) is recommended for detection of PERK of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-PERK Antibody (B-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PERK (B-5): sc-377400.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PERK Antibody (B-5) for WB and IHC(P) applications. These reagents are now offered in bundles with PERK Antibody (B-5) (see ordering information below).
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PERK Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 21-320 mapping near the N-terminus of human PERK and reacts with proteins from mouse, rat, and human origins; anti-PERK antibody (B-5) is suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry. PERK, also known as eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), is a type I transmembrane protein located in the endoplasmic reticulum (ER) that plays a critical role in cellular stress responses; PERK′s primary function is to detect the accumulation of misfolded proteins within the ER lumen and initiate the unfolded protein response (UPR) by phosphorylating the alpha subunit of eukaryotic initiation factor 2 (eIF2α). This phosphorylation event reduces general protein synthesis, thereby decreasing the influx of new proteins into the ER and allowing the cell to restore normal function, while selectively upregulating stress-related genes such as ATF4 that promote adaptation and survival under stress conditions. Understanding PERK′s function is important because dysregulation of the UPR and ER stress responses are implicated in various diseases, including neurodegenerative disorders, diabetes, and cancer; studying PERK can provide valuable insights into the mechanisms of these diseases and aid in the development of potential therapeutic targets. PERK monoclonal antibody (B-5) provides a valuable tool for researchers investigating PERK expression, localization, and activity in different cellular contexts, contributing to a deeper understanding of cellular homeostasis and stress management.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PERK Antibody (B-5) References:
- Characterization of a mutant pancreatic eIF-2alpha kinase, PEK, and co-localization with somatostatin in islet delta cells. | Shi, Y., et al. 1999. J Biol Chem. 274: 5723-30. PMID: 10026192
- Mechanism of interferon action: cDNA structure, expression, and regulation of the interferon-induced, RNA-dependent P1/eIF-2 alpha protein kinase from human cells. | Thomis, DC., et al. 1992. Virology. 188: 33-46. PMID: 1373553
- Mechanism of interferon action: identification of a RNA binding domain within the N-terminal region of the human RNA-dependent P1/eIF-2 alpha protein kinase. | McCormack, SJ., et al. 1992. Virology. 188: 47-56. PMID: 1373554
- Molecular cloning and characterization of the human double-stranded RNA-activated protein kinase induced by interferon. | Meurs, E., et al. 1990. Cell. 62: 379-90. PMID: 1695551
- Protein phosphorylation controls translation rates. | Hershey, JW. 1989. J Biol Chem. 264: 20823-6. PMID: 2687263
- The eIF-2 alpha protein kinases, regulators of translation in eukaryotes from yeasts to humans. | Samuel, CE. 1993. J Biol Chem. 268: 7603-6. PMID: 8096514
- Protein translation and folding are coupled by an endoplasmic-reticulum-resident kinase. | Harding, HP., et al. 1999. Nature. 397: 271-4. PMID: 9930704
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PERK Antibody (B-5) | sc-377400 | 200 µg/ml | $316.00 | |||
PERK Antibody (B-5): m-IgG Fc BP-HRP Bundle | sc-530021 | 200 µg Ab; 10 µg BP | $354.00 | |||
PERK Antibody (B-5): m-IgGκ BP-HRP Bundle | sc-523313 | 200 µg Ab, 40 µg BP | $354.00 | |||
PERK Antibody (B-5): m-IgG1 BP-HRP Bundle | sc-543822 | 200 µg Ab; 20 µg BP | $354.00 | |||
PERK Antibody (B-5) AC | sc-377400 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PERK Antibody (B-5) HRP | sc-377400 HRP | 200 µg/ml | $316.00 | |||
PERK Antibody (B-5) FITC | sc-377400 FITC | 200 µg/ml | $330.00 | |||
PERK Antibody (B-5) PE | sc-377400 PE | 200 µg/ml | $343.00 | |||
PERK Antibody (B-5) Alexa Fluor® 488 | sc-377400 AF488 | 200 µg/ml | $357.00 | |||
PERK Antibody (B-5) Alexa Fluor® 546 | sc-377400 AF546 | 200 µg/ml | $357.00 | |||
PERK Antibody (B-5) Alexa Fluor® 594 | sc-377400 AF594 | 200 µg/ml | $357.00 | |||
PERK Antibody (B-5) Alexa Fluor® 647 | sc-377400 AF647 | 200 µg/ml | $357.00 | |||
PERK Antibody (B-5) Alexa Fluor® 680 | sc-377400 AF680 | 200 µg/ml | $357.00 | |||
PERK Antibody (B-5) Alexa Fluor® 790 | sc-377400 AF790 | 200 µg/ml | $357.00 |