PERK Antibody (B-5): sc-377400

PERK Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 21-320 mapping near the N-terminus of human PERK and reacts with proteins from mouse, rat, and human origins; anti-PERK antibody (B-5) is suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry. PERK, also known as eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), is a type I transmembrane protein located in the endoplasmic reticulum (ER) that plays a critical role in cellular stress responses; PERK′s primary function is to detect the accumulation of misfolded proteins within the ER lumen and initiate the unfolded protein response (UPR) by phosphorylating the alpha subunit of eukaryotic initiation factor 2 (eIF2α). This phosphorylation event reduces general protein synthesis, thereby decreasing the influx of new proteins into the ER and allowing the cell to restore normal function, while selectively upregulating stress-related genes such as ATF4 that promote adaptation and survival under stress conditions. Understanding PERK′s function is important because dysregulation of the UPR and ER stress responses are implicated in various diseases, including neurodegenerative disorders, diabetes, and cancer; studying PERK can provide valuable insights into the mechanisms of these diseases and aid in the development of potential therapeutic targets. PERK monoclonal antibody (B-5) provides a valuable tool for researchers investigating PERK expression, localization, and activity in different cellular contexts, contributing to a deeper understanding of cellular homeostasis and stress management.

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PERK Antibody (B-5) References:

1. Characterization of a mutant pancreatic eIF-2alpha kinase, PEK, and co-localization with somatostatin in islet delta cells.  |  Shi, Y., et al. 1999. J Biol Chem. 274: 5723-30. PMID: 10026192
2. Mechanism of interferon action: cDNA structure, expression, and regulation of the interferon-induced, RNA-dependent P1/eIF-2 alpha protein kinase from human cells.  |  Thomis, DC., et al. 1992. Virology. 188: 33-46. PMID: 1373553
3. Mechanism of interferon action: identification of a RNA binding domain within the N-terminal region of the human RNA-dependent P1/eIF-2 alpha protein kinase.  |  McCormack, SJ., et al. 1992. Virology. 188: 47-56. PMID: 1373554
4. Molecular cloning and characterization of the human double-stranded RNA-activated protein kinase induced by interferon.  |  Meurs, E., et al. 1990. Cell. 62: 379-90. PMID: 1695551
5. Protein phosphorylation controls translation rates.  |  Hershey, JW. 1989. J Biol Chem. 264: 20823-6. PMID: 2687263
6. The eIF-2 alpha protein kinases, regulators of translation in eukaryotes from yeasts to humans.  |  Samuel, CE. 1993. J Biol Chem. 268: 7603-6. PMID: 8096514
7. Protein translation and folding are coupled by an endoplasmic-reticulum-resident kinase.  |  Harding, HP., et al. 1999. Nature. 397: 271-4. PMID: 9930704

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PERK Antibody (B-5) has 112 citations in a variety of scientific publications.

Primary antibodies like Anti-PERK Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PERK Antibody (B-5) Specifications

Alternate Product Name: PERK

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 21-320

Epitope Target: PERK

Epitope Species: human

Product Reactivity/Detection: PERK

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 112 citations in various scientific publications

Available Conjugates/Forms for PERK Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin