PDK4 Antibody (B-1): sc-518061

PDK4 Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects PDK4 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDK4 Antibody (B-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PDK4, a member of the pyruvate dehydrogenase kinase family, plays a crucial role in cellular metabolism by regulating the activity of the pyruvate dehydrogenase complex (PDC) through phosphorylation of its E1α subunit. This regulation is vital as PDK4 determines the balance between carbohydrate and fat metabolism, particularly during metabolic stress such as starvation or diabetes. PDK4 is predominantly expressed in heart and skeletal muscle, where PDK4 levels fluctuate in response to nutritional status; for instance, PDK4 levels increase during starvation, promoting fatty acid oxidation, and decrease upon re-feeding or insulin exposure, indicating PDK4′s importance in the long-term regulation of PDC activity. The structural integrity of PDK4, which contains five conserved regions and shares mechanistic similarities with bacterial His-kinases, is essential for function, as PDK4 requires specific histidine residues for catalytic activity. anti-PDK4 antibody (B-1) serves as a valuable tool for studying metabolic regulation and the physiological implications of PDK4 in human health and disease.

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PDK4 Antibody (B-1) References:

1. Histidine modifying agents abolish pyruvate dehydrogenase kinase activity.  |  Mooney, BP., et al. 2000. Biochem Biophys Res Commun. 267: 500-3. PMID: 10631090
2. Marked differences between two isoforms of human pyruvate dehydrogenase kinase.  |  Baker, JC., et al. 2000. J Biol Chem. 275: 15773-81. PMID: 10748134
3. Starvation increases the amount of pyruvate dehydrogenase kinase in several mammalian tissues.  |  Wu, P., et al. 2000. Arch Biochem Biophys. 381: 1-7. PMID: 11019813
4. Selective modification of the pyruvate dehydrogenase kinase isoform profile in skeletal muscle in hyperthyroidism: implications for the regulatory impact of glucose on fatty acid oxidation.  |  Sugden, MC., et al. 2000. J Endocrinol. 167: 339-45. PMID: 11054649
5. Cardiomyocyte Pdk4 response is associated with metabolic maladaptation in aging.  |  Fatmi, MK., et al. 2023. Aging Cell. 22: e13800. PMID: 36797808
6. The GR-PDK4 Axis of Evil: Hijacking a Stress SOS to Drive Metastasis.  |  Chakraborty, AA. and Keri, RA. 2023. Endocrinology. 164: PMID: 37459450
7. TUG1 protects against ferroptosis of hepatic stellate cells by upregulating PDK4-mediated glycolysis.  |  Zhang, X., et al. 2023. Chem Biol Interact. 383: 110673. PMID: 37582412
8. PDK4 inhibits osteoarthritis progression by activating the PPAR pathway.  |  Li, Z., et al. 2024. J Orthop Surg Res. 19: 109. PMID: 38308345
9. PDK4-mediated Nrf2 inactivation contributes to oxidative stress and diabetic kidney injury.  |  Tian, S., et al. 2024. Cell Signal. 121: 111282. PMID: 38971568
10. PDK4-mediated metabolic reprogramming is a potential therapeutic target for neovascular age-related macular degeneration.  |  Kim, J., et al. 2024. Cell Death Dis. 15: 582. PMID: 39122684
11. Diversity of the pyruvate dehydrogenase kinase gene family in humans.  |  Gudi, R., et al. 1995. J Biol Chem. 270: 28989-94. PMID: 7499431
12. Evidence for existence of tissue-specific regulation of the mammalian pyruvate dehydrogenase complex.  |  Bowker-Kinley, MM., et al. 1998. Biochem J. 329 (Pt 1): 191-6. PMID: 9405293

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PDK4 Antibody (B-1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PDK4 Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PDK4 Antibody (B-1) Specifications

Alternate Product Name: PDK4

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 14-38

Epitope Target: PDK4

Epitope Species: human

Product Reactivity/Detection: PDK4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PDK4 Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PDK4 antibody is PDK4 Antibody (A-7): sc-518103.