PDK4 Antibody (A-7): sc-518103
- PDK4 Antibody (A-7) is a mouse monoclonal IgG3 κ PDK4 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 386-407 near the C-terminus of PDK4 of human origin
- recommended for detection of PDK4 of mouse, rat and human origin by WB, IP, IF and ELISA; may cross-react with PDK1
- Anti-PDK4 Antibody (A-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG3 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PDK4 Antibody (A-7) for WB applications. These reagents are now offered in bundles with PDK4 Antibody (A-7) (see ordering information below).
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PDK4 Antibody (A-7) is a mouse monoclonal IgG3 kappa light chain antibody that detects PDK4 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PDK4 antibody (A-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PDK4, a member of the pyruvate dehydrogenase kinase family, plays a crucial role in cellular metabolism by regulating the activity of the pyruvate dehydrogenase complex (PDC) through phosphorylation of its E1α subunit. This regulation is vital as PDK4 determines the balance between carbohydrate and fatty acid oxidation, particularly during metabolic stress such as starvation or diabetes. PDK4 is predominantly expressed in heart and skeletal muscle, where PDK4 levels fluctuate in response to nutritional status; for instance, PDK4 levels increase during starvation, leading to reduced PDC activity and a shift towards fatty acid utilization for energy. Understanding PDK4 expression and activity is essential for elucidating metabolic adaptations in various physiological and pathological states, making PDK4 monoclonal antibody (A-7) a valuable tool for researchers studying metabolic regulation and related diseases.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PDK4 Antibody (A-7) References:
- Protein PDK4 Interacts with HMGCS2 to Facilitate High Glucoseinduced Myocardial Injuries. | Tan, W., et al. 2023. Curr Mol Med. 23: 1104-1115. PMID: 36281857
- SIRT6 inhibits hypoxia-induced pulmonary arterial smooth muscle cells proliferation via HIF-1α/PDK4 signaling. | Li, M., et al. 2023. Life Sci. 312: 121192. PMID: 36396113
- Glucocorticoid Receptors Drive Breast Cancer Cell Migration and Metabolic Reprogramming via PDK4. | Dwyer, AR., et al. 2023. Endocrinology. 164: PMID: 37224504
- PDK4 facilitates fibroblast functions and diabetic wound healing through regulation of HIF-1α protein stability and gene expression. | Ma, Z., et al. 2023. FASEB J. 37: e23215. PMID: 37737961
- High glucose promotes benign prostatic hyperplasia by downregulating PDK4 expression. | Wei, P., et al. 2023. Sci Rep. 13: 17910. PMID: 37863991
- A tryptophan-derived uremic metabolite/Ahr/Pdk4 axis governs skeletal muscle mitochondrial energetics in chronic kidney disease. | Thome, T., et al. 2024. JCI Insight. 9: PMID: 38652558
- Ginsenoside Rh2 shifts tumor metabolism from aerobic glycolysis to oxidative phosphorylation through regulating the HIF1-α/PDK4 axis in non-small cell lung cancer. | Liu, X., et al. 2024. Mol Med. 30: 56. PMID: 38671369
- Abrogating PDK4 activates autophagy-dependent ferroptosis in breast cancer via ASK1/JNK pathway. | Shi, W., et al. 2024. J Cancer Res Clin Oncol. 150: 218. PMID: 38678126
- Decreased AMPK/SIRT1/PDK4 induced by androgen excess inhibits human endometrial stromal cell decidualization in PCOS. | Hong, L., et al. 2024. Cell Mol Life Sci. 81: 324. PMID: 39080028
- Dichloroacetate Prevents Sepsis Associated Encephalopathy by Inhibiting Microglia Pyroptosis through PDK4/NLRP3. | Huang, X., et al. 2024. Inflammation.. PMID: 39177920
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDK4 Antibody (A-7) | sc-518103 | 200 µg/ml | $316.00 | |||
PDK4 Antibody (A-7): m-IgGκ BP-HRP Bundle | sc-525370 | 200 µg Ab, 40 µg BP | $354.00 | |||
PDK4 Antibody (A-7): m-IgG3 BP-HRP Bundle | sc-550368 | 200 µg Ab; 40 µg BP | $354.00 | |||
PDK4 Antibody (A-7) AC | sc-518103 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PDK4 Antibody (A-7) HRP | sc-518103 HRP | 200 µg/ml | $316.00 | |||
PDK4 Antibody (A-7) FITC | sc-518103 FITC | 200 µg/ml | $330.00 | |||
PDK4 Antibody (A-7) PE | sc-518103 PE | 200 µg/ml | $343.00 | |||
PDK4 Antibody (A-7) Alexa Fluor® 488 | sc-518103 AF488 | 200 µg/ml | $357.00 | |||
PDK4 Antibody (A-7) Alexa Fluor® 546 | sc-518103 AF546 | 200 µg/ml | $357.00 | |||
PDK4 Antibody (A-7) Alexa Fluor® 594 | sc-518103 AF594 | 200 µg/ml | $357.00 | |||
PDK4 Antibody (A-7) Alexa Fluor® 647 | sc-518103 AF647 | 200 µg/ml | $357.00 | |||
PDK4 Antibody (A-7) Alexa Fluor® 680 | sc-518103 AF680 | 200 µg/ml | $357.00 | |||
PDK4 Antibody (A-7) Alexa Fluor® 790 | sc-518103 AF790 | 200 µg/ml | $357.00 |