PDE6G/H Antibody (A-2): sc-166350
- PDE6G/H Antibody (A-2) is a mouse monoclonal IgG2a κ PDE6G/H antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1-87 representing full length PDE6G of human origin
- PDE6G/H Antibody (A-2) is recommended for detection of PDE6G and PDE6H of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PDE6G/H Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PDE6G/H Antibody (A-2) for WB applications. These reagents are now offered in bundles with PDE6G/H Antibody (A-2) (see ordering information below).
PDE6G/H Antibody (A-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects PDE6G/H protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PDE6G/H antibody (A-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PDE6G protein, also known as phosphodiesterase 6G cGMP-specific rod gamma, plays a crucial role in the visual signaling pathway by hydrolyzing cyclic guanosine monophosphate (cGMP), thereby regulating the phototransduction cascade in rod photoreceptors. This process is vital for converting light signals into electrical signals, which are then transmitted to the brain for visual perception. Mutations in the PDE6G gene can lead to significant visual impairments, highlighting the importance of this protein in maintaining normal vision. Similarly, PDE6H, or phosphodiesterase 6H cGMP-specific cone gamma, is essential for cone photoreceptor function and is composed of two catalytic chains and two inhibitory chains. Defects in the PDE6H gene are associated with retinal cone dystrophy 3, a condition that affects color vision and night vision. PDE6G/H monoclonal antibody (A-2) effectively detects these proteins, making this antibody a valuable tool for researchers studying visual signal transduction and related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PDE6G/H Antibody (A-2) References:
- A point mutation (W70A) in the rod PDE-gamma gene desensitizing and delaying murine rod photoreceptors. | Salchow, DJ., et al. 1999. Invest Ophthalmol Vis Sci. 40: 3262-7. PMID: 10586951
- Physical mapping of the rod cGMP-phosphodiesterase -subunit (PDE6G) gene to pig chromosome 12. | Yu, M., et al. 2003. Anim Genet. 34: 76-7. PMID: 12580796
- Analysis of PDE6D and PDE6G genes for generalised progressive retinal atrophy (gPRA) mutations in dogs. | Dekomien, G. and Epplen, JT. 2003. Genet Sel Evol. 35: 445-56. PMID: 12927076
- A substitution of G to C in the cone cGMP-phosphodiesterase gamma subunit gene found in a distinctive form of cone dystrophy. | Piri, N., et al. 2005. Ophthalmology. 112: 159-66. PMID: 15629837
- A phosphodiesterase inhibitor specific to a subset of bovine retinal cones. | Hamilton, SE. and Hurley, JB. 1990. J Biol Chem. 265: 11259-64. PMID: 2162841
- Retinal degeneration in mice lacking the gamma subunit of the rod cGMP phosphodiesterase. | Tsang, SH., et al. 1996. Science. 272: 1026-9. PMID: 8638127
- Isolation and chromosomal localization of the human cone cGMP phosphodiesterase gamma cDNA (PDE6H). | Shimizu-Matsumoto, A., et al. 1996. Genomics. 32: 121-4. PMID: 8786098
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDE6G/H Antibody (A-2) | sc-166350 | 200 µg/ml | $316.00 | |||
PDE6G/H Antibody (A-2): m-IgG Fc BP-HRP Bundle | sc-525761 | 200 µg Ab; 10 µg BP | $354.00 | |||
PDE6G/H Antibody (A-2): m-IgG2a BP-HRP Bundle | sc-546403 | 200 µg Ab; 10 µg BP | $354.00 | |||
PDE6G/H Antibody (A-2) AC | sc-166350 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PDE6G/H Antibody (A-2) HRP | sc-166350 HRP | 200 µg/ml | $316.00 | |||
PDE6G/H Antibody (A-2) FITC | sc-166350 FITC | 200 µg/ml | $330.00 | |||
PDE6G/H Antibody (A-2) PE | sc-166350 PE | 200 µg/ml | $343.00 | |||
PDE6G/H Antibody (A-2) Alexa Fluor® 488 | sc-166350 AF488 | 200 µg/ml | $357.00 | |||
PDE6G/H Antibody (A-2) Alexa Fluor® 546 | sc-166350 AF546 | 200 µg/ml | $357.00 | |||
PDE6G/H Antibody (A-2) Alexa Fluor® 594 | sc-166350 AF594 | 200 µg/ml | $357.00 | |||
PDE6G/H Antibody (A-2) Alexa Fluor® 647 | sc-166350 AF647 | 200 µg/ml | $357.00 | |||
PDE6G/H Antibody (A-2) Alexa Fluor® 680 | sc-166350 AF680 | 200 µg/ml | $357.00 | |||
PDE6G/H Antibody (A-2) Alexa Fluor® 790 | sc-166350 AF790 | 200 µg/ml | $357.00 |