PAR-3 Antibody (G-4): sc-393127

PAR-3 Antibody (G-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects PAR-3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PAR-3 Antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PAR-3, also known as protease-activated receptor 3, plays a crucial role in signaling pathways activated by thrombin, a serine protease essential for blood coagulation and platelet aggregation. Upon activation, PAR-3 influences cellular responses such as mitogenesis and thrombosis, highlighting PAR-3′s importance in vascular biology and hemostasis. PAR-3 is primarily located in the cell membrane, where PAR-3 functions as a G protein-coupled receptor, facilitating communication between extracellular signals and intracellular responses. This localization is vital for PAR-3′s role in mediating effects of thrombin and other proteases, which can lead to significant physiological outcomes, including inflammation and tissue repair. Understanding PAR-3′s function and location is essential for elucidating PAR-3′s contributions to various pathophysiological conditions, including cardiovascular diseases and inflammatory responses.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PAR-3 Antibody (G-4) References:

1. The Par-3 NTD adopts a PB1-like structure required for Par-3 oligomerization and membrane localization.  |  Feng, W., et al. 2007. EMBO J. 26: 2786-96. PMID: 17476308
2. Phosphoinositide binding by par-3 involved in par-3 localization.  |  Horikoshi, Y., et al. 2011. Cell Struct Funct. 36: 97-102. PMID: 21467691
3. Loss of Par3 promotes lung adenocarcinoma metastasis through 14-3-3ζ protein.  |  Song, T., et al. 2016. Oncotarget. 7: 64260-64273. PMID: 27588399
4. Flow cytometry analysis reveals different activation profiles of thrombin- or TRAP-stimulated platelets in db/db mice. The regulatory role of PAR-3.  |  Kassassir, H., et al. 2017. Blood Cells Mol Dis. 65: 16-22. PMID: 28460264
5. PAR-3 controls endothelial planar polarity and vascular inflammation under laminar flow.  |  Hikita, T., et al. 2018. EMBO Rep. 19: PMID: 30018153
6. A Conserved PDZ-Binding Motif in aPKC Interacts with Par-3 and Mediates Cortical Polarity.  |  Holly, RW., et al. 2020. Curr Biol. 30: 893-898.e5. PMID: 32084408
7. Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division.  |  Zhao, X., et al. 2021. Sci Adv. 7: PMID: 34117063
8. A particle size threshold governs diffusion and segregation of PAR-3 during cell polarization.  |  Chang, Y. and Dickinson, DJ. 2022. Cell Rep. 39: 110652. PMID: 35417695
9. The Roles of Par3, Par6, and aPKC Polarity Proteins in Normal Neurodevelopment and in Neurodegenerative and Neuropsychiatric Disorders.  |  Zhang, L. and Wei, X. 2022. J Neurosci. 42: 4774-4793. PMID: 35705493
10. EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks.  |  Banerjee, SL., et al. 2022. Cell Rep. 40: 111031. PMID: 35793621

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PAR-3 Antibody (G-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PAR-3 Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PAR-3 Antibody (G-4) Specifications

Alternate Product Name: Proteinase-Activated Receptor 3

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-103

Epitope Target: PAR-3

Epitope Species: human

Product Reactivity/Detection: PAR-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PAR-3 Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PAR-3 antibody is PAR-3 Antibody (8E8): sc-53819.