PAR-3 Antibody (8E8): sc-53819

PAR-3 Antibody (8E8) is a mouse monoclonal IgG2b kappa light chain antibody that detects PAR-3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). Anti-PAR-3 antibody (8E8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. PAR-3, a member of the protease-activated receptor family, plays a crucial role in mediating cellular responses to thrombin, a key serine protease involved in blood coagulation and platelet activation. PAR-3 is primarily located on the cell surface, where PAR-3 functions as a G protein-coupled receptor, facilitating signal transduction pathways that lead to increased intracellular calcium levels and subsequent cellular responses. This localization allows PAR-3 to rapidly respond to proteolytic cleavage by thrombin, thereby influencing processes such as thrombosis and mitogenesis. PAR-3′s interaction with other receptors, such as PAR-1 and PAR-2, further underscores PAR-3′s importance in the complex signaling networks that regulate hemostasis and inflammation. Understanding PAR-3′s function and location is essential for elucidating PAR-3′s role in various physiological and pathological processes, making PAR-3 monoclonal antibody (8E8) an invaluable tool for researchers studying these mechanisms.

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PAR-3 Antibody (8E8) References:

1. Protease-activated receptors 1 and 4 mediate activation of human platelets by thrombin.  |  Kahn, ML., et al. 1999. J Clin Invest. 103: 879-87. PMID: 10079109
2. PAR3-PAR6-atypical PKC polarity complex proteins in neuronal polarization.  |  Hapak, SM., et al. 2018. Cell Mol Life Sci. 75: 2735-2761. PMID: 29696344
3. Par-3 family proteins in cell polarity & adhesion.  |  Thompson, BJ. 2022. FEBS J. 289: 596-613. PMID: 33565714
4. Dual Function of Par3 in Tumorigenesis.  |  Lv, T., et al. 2022. Front Oncol. 12: 915957. PMID: 35875120
5. Negative cooperativity underlies dynamic assembly of the Par complex regulators Cdc42 and Par-3.  |  Vargas, E. and Prehoda, KE. 2023. J Biol Chem. 299: 102749. PMID: 36436559
6. Evidence for the presence of a protease-activated receptor distinct from the thrombin receptor in human keratinocytes.  |  Santulli, RJ., et al. 1995. Proc Natl Acad Sci U S A. 92: 9151-5. PMID: 7568091
7. Agonist recognition by proteinase-activated receptor 2 and thrombin receptor. Importance of extracellular loop interactions for receptor function.  |  Lerner, DJ., et al. 1996. J Biol Chem. 271: 13943-7. PMID: 8662993
8. The proteinase-activated receptor 2 is induced by inflammatory mediators in human endothelial cells. Comparison with the thrombin receptor.  |  Nystedt, S., et al. 1996. J Biol Chem. 271: 14910-5. PMID: 8663011
9. The human proteinase-activated receptor-3 (PAR-3) gene. Identification within a Par gene cluster and characterization in vascular endothelial cells and platelets.  |  Schmidt, VA., et al. 1998. J Biol Chem. 273: 15061-8. PMID: 9614115
10. Cloning and characterization of human protease-activated receptor 4.  |  Xu, WF., et al. 1998. Proc Natl Acad Sci U S A. 95: 6642-6. PMID: 9618465
11. Thrombin.  |  Goldsack, NR., et al. 1998. Int J Biochem Cell Biol. 30: 641-6. PMID: 9695019
12. Analysis of a Ca2+-activated K+ channel that mediates hyperpolarization via the thrombin receptor pathway.  |  Sullivan, R., et al. 1998. Am J Physiol. 275: C1342-8. PMID: 9814983

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PAR-3 Antibody (8E8) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-PAR-3 Antibody (8E8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PAR-3 Antibody (8E8) Specifications

Alternate Product Name: Proteinase-Activated Receptor 3

Product Clone Name: 8E8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 31-47

Epitope Target: PAR-3

Epitope Species: human

Product Reactivity/Detection: PAR-3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for PAR-3 Antibody (8E8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PAR-3 antibody is PAR-3 Antibody (G-4): sc-393127.