PAI-1/SERPINE1 Antibody (C-9): sc-5297

PAI-1 Antibody (C-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects PAI-1 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PAI-1 Antibody (C-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PAI-1, also known as SERPINE1, plays a crucial role in regulating fibrinolysis and tissue remodeling by inhibiting urokinase-type plasminogen activator (uPA) and tissue plasminogen activator (tPA), which are essential for the conversion of plasminogen to plasmin. This regulation is vital for maintaining hemostasis and preventing excessive tissue degradation, making PAI-1 a significant factor in various physiological and pathological processes, including wound healing, cancer progression, and cardiovascular diseases. PAI-1 can exist in both active and latent forms, with the latent form being able to regain activity through denaturation and renaturation, highlighting PAI-1′s dynamic role in the regulation of proteolytic activity. The ability of PAI-1 to fold into an inactive state without cleavage underscores the complexity of PAI-1′s function and importance in cellular processes.

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PAI-1/SERPINE1 Antibody (C-9) References:

1. Structural basis of latency in plasminogen activator inhibitor-1.  |  Mottonen, J., et al. 1992. Nature. 355: 270-3. PMID: 1731226
2. Human tumor cell urokinase-type plasminogen activator (uPA): degradation of the proenzyme form (pro-uPA) by granulocyte elastase prevents subsequent activation by plasmin.  |  Schmitt, M., et al. 1991. Adv Exp Med Biol. 297: 111-28. PMID: 1837419
3. Facultative polypeptide translocation allows a single mRNA to encode the secreted and cytosolic forms of plasminogen activators inhibitor 2.  |  Belin, D., et al. 1989. EMBO J. 8: 3287-94. PMID: 2583099
4. The human urokinase-plasminogen activator gene and its promoter.  |  Riccio, A., et al. 1985. Nucleic Acids Res. 13: 2759-71. PMID: 2987867
5. The Central Role of PAI-1 in COVID-19: Thrombosis and beyond.  |  Khan, SS. 2021. Am J Respir Cell Mol Biol. 65: 238-240. PMID: 34086538
6. Glomerular endothelial cell senescence drives age-related kidney disease through PAI-1.  |  Cohen, C., et al. 2021. EMBO Mol Med. 13: e14146. PMID: 34725920
7. PAI-1 Drives Septation and Clinical Outcomes in Pleural Infection.  |  Komissarov, AA. and Idell, S. 2023. Am J Respir Crit Care Med. 207: 653-655. PMID: 36269762
8. PAI-1 mediates TGF-β1-induced myofibroblast activation in tenocytes via mTOR signaling.  |  Alenchery, RG., et al. 2023. J Orthop Res. 41: 2163-2174. PMID: 37143206
9. PAI-1 regulates AT2-mediated re-alveolarization and ion permeability.  |  Ali, G., et al. 2023. Stem Cell Res Ther. 14: 185. PMID: 37501095
10. Intracellular tPA-PAI-1 interaction determines VLDL assembly in hepatocytes.  |  Dai, W., et al. 2023. Science. 381: eadh5207. PMID: 37651538
11. Differential expression of urokinase-type plasminogen activator (uPA), its receptor (uPA-R), and inhibitor type-2 (PAI-2) during differentiation of keratinocytes in an organotypic coculture system.  |  Schaefer, BM., et al. 1995. Exp Cell Res. 220: 415-23. PMID: 7556451
12. Cytokine regulation of endothelial cell extracellular proteolysis.  |  Niedbala, MJ. 1993. Agents Actions Suppl. 42: 179-93. PMID: 8356923

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PAI-1/SERPINE1 Antibody (C-9) has 123 citations in a variety of scientific publications.

Primary antibodies like Anti-PAI-1/SERPINE1 Antibody (C-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PAI-1/SERPINE1 Antibody (C-9) Specifications

Alternate Product Name: PAI-1

Product Clone Name: C-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 24-158

Epitope Target: PAI-1

Epitope Species: human

Product Reactivity/Detection: PAI-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 123 citations in various scientific publications

Available Conjugates/Forms for PAI-1/SERPINE1 Antibody (C-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin