p63α Antibody (C-12): sc-5301

p63α Antibody (C-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects the human isoforms tA* p63α and ΔN p63α through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and ELISA. p63α monoclonal antibody (C-12) is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and various Alexa Fluor® conjugates. p63, a member of the p53 gene family, plays a crucial role as a transcription factor and is predominantly located in the nucleus, where p63 exists as twelve isoforms due to alternative splicing. The two main variants, TAp63 and ΔNp63, are produced from different promoters, with TAp63 being associated with transactivation similar to p53, while ΔNp63 exhibits distinct biological functions and is known to be oncogenic. p63 presence in the nucleus is vital for regulating gene expression related to cell growth, survival, and differentiation. ΔNp63 is essential for cell proliferation and can exert a dominant-negative effect over TAp63 and p53, highlighting ΔNp63 importance in cancer biology. TAp63 is involved in processes such as tubulogenesis and cyst formation, underscoring the diverse roles of p63 in cellular functions. Anti-p63α monoclonal antibody (C-12) is suitable for use in various research applications, making p63α (C-12) monoclonal antibody an invaluable tool for studying the complex biology of p63 and its implications in health and disease.

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p63α Antibody (C-12) References:

1. Constitutive expression of DeltaN-p63alpha isoform in human thymus and thymic epithelial tumours.  |  Chilosi, M., et al. 2003. Virchows Arch. 443: 175-83. PMID: 12851817
2. Altered sumoylation of p63alpha contributes to the split-hand/foot malformation phenotype.  |  Huang, YP., et al. 2004. Cell Cycle. 3: 1587-96. PMID: 15539951
3. Unique domain functions of p63 isotypes that differentially regulate distinct aspects of epidermal homeostasis.  |  King, KE., et al. 2006. Carcinogenesis. 27: 53-63. PMID: 16081516
4. Overexpression and ratio disruption of ΔNp63 and TAp63 isoform equilibrium in endometrial adenocarcinoma: correlation with obesity, menopause, and grade I/II tumors.  |  Vakonaki, E., et al. 2012. J Cancer Res Clin Oncol. 138: 1271-8. PMID: 22441934
5. P63 regulates tubular formation via epithelial-to-mesenchymal transition.  |  Zhang, Y., et al. 2014. Oncogene. 33: 1548-57. PMID: 23542170
6. p63-expressing cells are the stem cells of developing prostate, bladder, and colorectal epithelia.  |  Pignon, JC., et al. 2013. Proc Natl Acad Sci U S A. 110: 8105-10. PMID: 23620512
7. Transcription factor p63 regulates key genes and wound repair in human airway epithelial basal cells.  |  Warner, SM., et al. 2013. Am J Respir Cell Mol Biol. 49: 978-88. PMID: 23837456
8. p63α modulates c-Myc activity via direct interaction and regulation of MM1 protein stability.  |  Han, A., et al. 2016. Oncotarget. 7: 44277-44287. PMID: 27341130
9. PABPN1, a Target of p63, Modulates Keratinocyte Differentiation through Regulation of p63α mRNA Translation.  |  Mohibi, S., et al. 2020. J Invest Dermatol. 140: 2166-2177.e6. PMID: 32243883
10. The p63 C-terminus is essential for murine oocyte integrity.  |  Lena, AM., et al. 2021. Nat Commun. 12: 383. PMID: 33452256
11. Characterization of Porcine Ocular Surface Epithelial Microenvironment.  |  Polisetti, N., et al. 2023. Int J Mol Sci. 24: PMID: 37108705
12. Two new p73 splice variants, gamma and delta, with different transcriptional activity.  |  De Laurenzi, V., et al. 1998. J Exp Med. 188: 1763-8. PMID: 9802988

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p63α Antibody (C-12) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-p63α Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p63α Antibody (C-12) Specifications

Alternate Product Name: TP63

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 513-641

Epitope Target: TA* p63α

Epitope Species: human

Product Reactivity/Detection: tA* p63α and ΔN p63α

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for p63α Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p63 antibody is p63 Antibody (D-9): sc-25268.