p63 Antibody (D-9): sc-25268

p63 Antibody (D-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects p63 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-p63 antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p63, a member of the p53 gene family, plays a crucial role in regulating epithelial development and maintaining epithelial tissue integrity. p63 exists as twelve isoforms, primarily TAp63 and ΔNp63, which arise from alternate promoter usage and splicing events. TAp63 transactivates p53 target genes, contributing to processes such as tubulogenesis and cyst formation, while ΔNp63, often considered oncogenic, is vital for cell growth and survival, exerting a dominant-negative effect over TAp63 and p53. The diverse functions of p63 isoforms underscore their importance in both normal cellular processes and cancer biology, making p63 (D-9) monoclonal antibody an invaluable tool for researchers studying these critical pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p63 Antibody (D-9) References:

1. Unique domain functions of p63 isotypes that differentially regulate distinct aspects of epidermal homeostasis.  |  King, KE., et al. 2006. Carcinogenesis. 27: 53-63. PMID: 16081516
2. Overexpression and ratio disruption of ΔNp63 and TAp63 isoform equilibrium in endometrial adenocarcinoma: correlation with obesity, menopause, and grade I/II tumors.  |  Vakonaki, E., et al. 2012. J Cancer Res Clin Oncol. 138: 1271-8. PMID: 22441934
3. P63 regulates tubular formation via epithelial-to-mesenchymal transition.  |  Zhang, Y., et al. 2014. Oncogene. 33: 1548-57. PMID: 23542170
4. p63-expressing cells are the stem cells of developing prostate, bladder, and colorectal epithelia.  |  Pignon, JC., et al. 2013. Proc Natl Acad Sci U S A. 110: 8105-10. PMID: 23620512
5. Transcription factor p63 regulates key genes and wound repair in human airway epithelial basal cells.  |  Warner, SM., et al. 2013. Am J Respir Cell Mol Biol. 49: 978-88. PMID: 23837456
6. p63(+)Krt5(+) distal airway stem cells are essential for lung regeneration.  |  Zuo, W., et al. 2015. Nature. 517: 616-20. PMID: 25383540
7. p63 in corneal and epidermal differentiation.  |  Novelli, F., et al. 2022. Biochem Biophys Res Commun. 610: 15-22. PMID: 35430447
8. Hepatic p63 regulates glucose metabolism by repressing SIRT1.  |  Gonzalez-Rellan, MJ., et al. 2023. Gut. 72: 472-483. PMID: 35580962
9. STAT3 and p63 in the Regulation of Cancer Stemness.  |  Wei, S., et al. 2022. Front Genet. 13: 909251. PMID: 36061200
10. The dual role of p63 in cancer.  |  Xu, Y., et al. 2023. Front Oncol. 13: 1116061. PMID: 37182132
11. Autologous transplantation of P63+ lung progenitor cells for chronic obstructive pulmonary disease therapy.  |  Wang, Y., et al. 2024. Sci Transl Med. 16: eadi3360. PMID: 38354225
12. Two new p73 splice variants, gamma and delta, with different transcriptional activity.  |  De Laurenzi, V., et al. 1998. J Exp Med. 188: 1763-8. PMID: 9802988

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p63 Antibody (D-9) has 55 citations in a variety of scientific publications.

Primary antibodies like Anti-p63 Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p63 Antibody (D-9) Specifications

Alternate Product Name: TP63

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 15-151

Epitope Target: ΔN p63α

Epitope Species: human

Product Reactivity/Detection: all p63 isoforms

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 55 citations in various scientific publications

Available Conjugates/Forms for p63 Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p63α antibody is p63α Antibody (C-12): sc-5301.