p53R2 Antibody (B-10): sc-137175

p53R2 Antibody (B-10) is a mouse monoclonal IgG2b kappa light chain antibody, designed to detect p53R2, also known as RRM2B, across mouse, rat, and human species. anti-p53R2 antibody (B-10) targets an epitope comprising amino acids 52-351 at the C-terminus of human p53R2, ensuring precise and reliable binding for various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p53R2 is a crucial subunit of ribonucleotide reductase, an enzyme essential for the synthesis of deoxyribonucleoside triphosphates (dNTPs) necessary for DNA repair and replication. Structurally, p53R2 forms part of the ribonucleotide reductase complex, collaborating with the R1 subunit to regulate dNTP production, particularly during the S-phase of the cell cycle and in response to DNA damage. Post-translational modifications of p53R2, such as phosphorylation, play a vital role in regulation and localization, enabling p53R2 to translocate to the nucleus upon genotoxic stress where p53R2 supplies an immediate pool of dNTPs for DNA repair processes. This dynamic regulation is essential for maintaining genomic stability and preventing mutations that could lead to cancer. p53R2 (B-10) antibody facilitates advanced research into cell cycle regulation, DNA damage response, and the molecular mechanisms underlying tumor suppression by providing reliable detection. p53R2 monoclonal antibody (B-10) is an indispensable tool for scientists investigating the structural and functional dynamics of ribonucleotide reductase in various biological contexts.

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p53R2 Antibody (B-10) References:

1. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage.  |  Tanaka, H., et al. 2000. Nature. 404: 42-9. PMID: 10716435
2. Controlled protein degradation regulates ribonucleotide reductase activity in proliferating mammalian cells during the normal cell cycle and in response to DNA damage and replication blocks.  |  Chabes, A. and Thelander, L. 2000. J Biol Chem. 275: 17747-53. PMID: 10747958
3. Definition of a consensus binding site for p53.  |  el-Deiry, WS., et al. 1992. Nat Genet. 1: 45-9. PMID: 1301998
4. S-phase-specific expression of mammalian ribonucleotide reductase R1 and R2 subunit mRNAs.  |  Björklund, S., et al. 1990. Biochemistry. 29: 5452-8. PMID: 1696835
5. Circular dichroism and magnetic circular dichroism studies of the active site of p53R2 from human and mouse: iron binding and nature of the biferrous site relative to other ribonucleotide reductases.  |  Wei, PP., et al. 2006. Biochemistry. 45: 14043-51. PMID: 17115699
6. Ribonucleotide reductase small subunit p53R2 facilitates p21 induction of G1 arrest under UV irradiation.  |  Xue, L., et al. 2007. Cancer Res. 67: 16-21. PMID: 17210678
7. Ribonucleotide reductase subunits M2 and p53R2 are potential biomarkers for metastasis of colon cancer.  |  Liu, X., et al. 2007. Clin Colorectal Cancer. 6: 374-81. PMID: 17311703
8. p53-inducible ribonucleotide reductase (p53R2/RRM2B) is a DNA hypomethylation-independent decitabine gene target that correlates with clinical response in myelodysplastic syndrome/acute myelogenous leukemia.  |  Link, PA., et al. 2008. Cancer Res. 68: 9358-66. PMID: 19010910
9. p53R2 is a prognostic factor of melanoma and regulates proliferation and chemosensitivity of melanoma cells.  |  Matsushita, S., et al. 2012. J Dermatol Sci. 68: 19-24. PMID: 22902076
10. Tumor suppressor FOXO3 regulates ribonucleotide reductase subunit RRM2B and impacts on survival of cancer patients.  |  Cho, EC., et al. 2014. Oncotarget. 5: 4834-44. PMID: 24947616
11. Mutations in the p53 tumor suppressor gene: clues to cancer etiology and molecular pathogenesis.  |  Greenblatt, MS., et al. 1994. Cancer Res. 54: 4855-78. PMID: 8069852
12. p53, the cellular gatekeeper for growth and division.  |  Levine, AJ. 1997. Cell. 88: 323-31. PMID: 9039259

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p53R2 Antibody (B-10) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-p53R2 Antibody (B-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p53R2 Antibody (B-10) Specifications

Alternate Product Name: p53R2

Product Clone Name: B-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 52-351

Epitope Target: p53R2

Epitope Species: human

Product Reactivity/Detection: p53R2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for p53R2 Antibody (B-10): N/A

The preferred R2 antibody is R2 Antibody (A-5): sc-398294.