p40-phox Antibody (B-1): sc-48376

p40-phox Antibody (B-1) is a mouse monoclonal IgG2b antibody that detects p40-phox in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p40-phox plays a crucial role in the activation of the NADPH-oxidase complex, which is essential for the production of reactive oxygen species during the immune response. p40-phox is primarily located in the cytosol and translocates to the membrane upon neutrophil stimulation, where p40-phox interacts with other cytosolic components like p47-phox and p67-phox to form a functional oxidase complex. The proper localization of p40-phox is vital for function, as p40-phox facilitates the assembly of the NADPH-oxidase complex, thereby enhancing the production of superoxide and other reactive intermediates that are critical for microbial killing. Additionally, p40-phox is subject to post-translational modifications, including phosphorylation at specific sites, which further regulate activity and interaction with other proteins. p40-phox Antibody (B-1) is an invaluable tool for researchers studying the mechanisms of oxidative stress and immune responses, providing insights into the dynamics of NADPH-oxidase activation and implications in various diseases.

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p40-phox Antibody (B-1) References:

1. Phosphorylation of p40-phox during activation of neutrophil NADPH oxidase.  |  Someya, A., et al. 1999. J Leukoc Biol. 66: 851-7. PMID: 10577519
2. p40(phox) Participates in the activation of NADPH oxidase by increasing the affinity of p47(phox) for flavocytochrome b(558).  |  Cross, AR. 2000. Biochem J. 349: 113-7. PMID: 10861218
3. Relationships of p40(phox) with p67(phox) in the activation and expression of the human respiratory burst NADPH oxidase.  |  Tsunawaki, S. and Yoshikawa, K. 2000. J Biochem. 128: 777-83. PMID: 11056390
4. The NADPH oxidase components p47(phox) and p40(phox) bind to moesin through their PX domain.  |  Wientjes, FB., et al. 2001. Biochem Biophys Res Commun. 289: 382-8. PMID: 11716484
5. The adaptor protein p40(phox) as a positive regulator of the superoxide-producing phagocyte oxidase.  |  Kuribayashi, F., et al. 2002. EMBO J. 21: 6312-20. PMID: 12456638
6. Knockout of p47 phox uncovers a critical role of p40 phox in reactive oxygen species production in microvascular endothelial cells.  |  Fan, LM., et al. 2009. Arterioscler Thromb Vasc Biol. 29: 1651-6. PMID: 19608974
7. Cooperation of p40(phox) with p47(phox) for Nox2-based NADPH oxidase activation during Fcγ receptor (FcγR)-mediated phagocytosis: mechanism for acquisition of p40(phox) phosphatidylinositol 3-phosphate (PI(3)P) binding.  |  Ueyama, T., et al. 2011. J Biol Chem. 286: 40693-705. PMID: 21956105
8. p40(phox) down-regulates NADPH oxidase activity through interactions with its SH3 domain.  |  Sathyamoorthy, M., et al. 1997. J Biol Chem. 272: 9141-6. PMID: 9083043
9. p40(phox) is phosphorylated on threonine 154 and serine 315 during activation of the phagocyte NADPH oxidase. Implication of a protein kinase c-type kinase in the phosphorylation process.  |  Bouin, AP., et al. 1998. J Biol Chem. 273: 30097-103. PMID: 9804763

The preferred p40-phox antibody is p40-phox Antibody (D-8): sc-48388.