Anti-p40-phox Antibody (D-8) recommended for detection of p40-phox of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-p40-phox Antibody (D-8): sc-48388

Anti-p40-phox Antibody (D-8) is rated 4.7 out of 5 by 7.
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Datasheets

    • Anti-p40-phox Antibody (D-8) is a mouse monoclonal IgG2b κ p40-phox antibody, cited in 12 publications, provided at 200 µg/ml
    • raised against amino acids 1-300 p40-phox of human origin
    • Anti-p40-phox Antibody (D-8) is recommended for detection of p40-phox of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-p40-phox Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p40-phox (D-8): sc-48388.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p40-phox Antibody (D-8) for WB and IHC(P) applications. These reagents are now offered in bundles with p40-phox Antibody (D-8) (see ordering information below).
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I am using p40-phox (D-8): sc-48388 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from worked great for western blot worked great for IP. highly recommended for experiment
Date published: 2019-12-04
Rated 4 out of 5 by from Worked well in WB with human cells Worked well in WB with human cells.
Date published: 2015-09-23
Rated 5 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2015-07-10
Rated 5 out of 5 by from Used for western blot analysis using human Used for western blot analysis using human CD34+ and murine Sca-1+ hematopoietic progenitor cells. -SCBT Publication Review
Date published: 2015-06-25
Rated 5 out of 5 by from Published WB data using WBCs Published WB data using WBCs. -SCBT Publication Review
Date published: 2015-05-27
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human bone marrow tissue. -SCBT QC
Date published: 2015-02-07
Rated 5 out of 5 by from WB: strong WB: strong, clean bands in human peripheral blood and HL-60 whole cell lysates. -SCBT QC
Date published: 2015-01-18
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