p40-phox Antibody (D-8): sc-48388

p40-phox Antibody (D-8) is a mouse monoclonal IgG2b kappa light chain antibody that detects p40-phox of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-p40-phox antibody (D-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p40-phox plays a crucial role in activating the NADPH oxidase complex, essential for producing reactive oxygen species during immune response. p40-phox is primarily located in the cytosol and translocates to the membrane upon neutrophil activation, where p40-phox interacts with other cytosolic components such as p47-phox and p67-phox to form a functional oxidase complex. Proper localization and interaction of p40-phox are vital for effective superoxide generation, critical for microbial killing and inflammation. p40-phox undergoes post-translational modifications, including phosphorylation at specific sites, which regulate activity and interaction with other proteins in the NADPH oxidase complex. Understanding p40-phox dynamics and role in immune response can provide insights into various inflammatory diseases and potential therapeutic targets.

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p40-phox Antibody (D-8) References:

1. Phosphorylation of p40-phox during activation of neutrophil NADPH oxidase.  |  Someya, A., et al. 1999. J Leukoc Biol. 66: 851-7. PMID: 10577519
2. p40(phox) Participates in the activation of NADPH oxidase by increasing the affinity of p47(phox) for flavocytochrome b(558).  |  Cross, AR. 2000. Biochem J. 349: 113-7. PMID: 10861218
3. Involvement of cytosolic prolyl endopeptidase in degradation of p40-phox splice variant protein in myeloid cells.  |  Hasebe, T., et al. 2001. J Leukoc Biol. 69: 963-8. PMID: 11404383
4. The NADPH oxidase components p47(phox) and p40(phox) bind to moesin through their PX domain.  |  Wientjes, FB., et al. 2001. Biochem Biophys Res Commun. 289: 382-8. PMID: 11716484
5. Vav proteins in neutrophils are required for FcgammaR-mediated signaling to Rac GTPases and nicotinamide adenine dinucleotide phosphate oxidase component p40(phox).  |  Utomo, A., et al. 2006. J Immunol. 177: 6388-97. PMID: 17056570
6. Cooperation of p40(phox) with p47(phox) for Nox2-based NADPH oxidase activation during Fcγ receptor (FcγR)-mediated phagocytosis: mechanism for acquisition of p40(phox) phosphatidylinositol 3-phosphate (PI(3)P) binding.  |  Ueyama, T., et al. 2011. J Biol Chem. 286: 40693-705. PMID: 21956105
7. Activation of neutrophil respiratory burst by fungal particles requires phosphatidylinositol 3-phosphate binding to p40(phox) in humans but not in mice.  |  Bagaitkar, J., et al. 2012. Blood. 120: 3385-7. PMID: 23086626
8. p40 phox -Deficient Mice Exhibit Impaired Bacterial Clearance and Enhanced Pro-inflammatory Responses during Salmonella enterica serovar Typhimurium Infection.  |  Li, Y., et al. 2017. Front Immunol. 8: 1270. PMID: 29062317
9. A New Patient with p40phox Deficiency and Chronic Immune Thrombocytopenia.  |  Neehus, AL., et al. 2023. J Clin Immunol. 43: 1173-1177. PMID: 37198372
10. Genomic structure, chromosomal localization, start of transcription, and tissue expression of the human p40-phox, a new component of the nicotinamide adenine dinucleotide phosphate-oxidase complex.  |  Zhan, S., et al. 1996. Blood. 88: 2714-21. PMID: 8839867
11. p40(phox) down-regulates NADPH oxidase activity through interactions with its SH3 domain.  |  Sathyamoorthy, M., et al. 1997. J Biol Chem. 272: 9141-6. PMID: 9083043
12. p40(phox) is phosphorylated on threonine 154 and serine 315 during activation of the phagocyte NADPH oxidase. Implication of a protein kinase c-type kinase in the phosphorylation process.  |  Bouin, AP., et al. 1998. J Biol Chem. 273: 30097-103. PMID: 9804763

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p40-phox Antibody (D-8) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-p40-phox Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p40-phox Antibody (D-8) Specifications

Alternate Product Name: NCF4

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-300

Epitope Target: p40-phox

Epitope Species: human

Product Reactivity/Detection: p40-phox

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for p40-phox Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin