p-ZAP-70 Antibody (pY319.17A): sc-136248
- p-ZAP-70 Antibody (pY319.17A) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- raised against a short amino acid sequence containing phosphorylated Tyr 319 of ZAP-70 of human origin
- recommended for detection of Tyr 319 phosphorylated ZAP-70 and Tyr 352 phosphorylated Syk of mouse, rat and human origin by WB and IP
- m-IgGκ BP-HRP is the preferred secondary detection reagent for p-ZAP-70 Antibody (pY319.17A) for WB applications. This reagent is now offered in a bundle with p-ZAP-70 Antibody (pY319.17A) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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p-ZAP-70 Antibody (pY319.17A) is a mouse monoclonal IgG1 antibody that detects p-ZAP-70 in mouse, rat, and human samples through applications such as western blotting (WB) and immunoprecipitation (IP). p-ZAP-70, a phosphorylated form of protein tyrosine kinase ZAP-70, plays a crucial role in T cell receptor signaling, which is essential for T lymphocyte activation and immune response. The phosphorylation of ZAP-70 at tyrosine 319 enhances the interaction between ZAP-70 and Lck, another key protein involved in T cell signaling. This interaction is vital for the propagation of signals that lead to T cell activation, proliferation, and differentiation, making p-ZAP-70 a critical player in the immune system′s ability to respond to pathogens. Anti-p-ZAP-70 antibody (pY319.17A) specifically recognizes this phosphorylated form, enabling researchers to study the dynamics of T cell activation and the underlying mechanisms of immune responses, providing valuable insights into immunological diseases and potential therapeutic targets.
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p-ZAP-70 Antibody (pY319.17A) References:
- Tyrosine 319, a newly identified phosphorylation site of ZAP-70, plays a critical role in T cell antigen receptor signaling. | Di Bartolo, V., et al. 1999. J Biol Chem. 274: 6285-94. PMID: 10037717
- Tyrosine 319 in the interdomain B of ZAP-70 is a binding site for the Src homology 2 domain of Lck. | Pelosi, M., et al. 1999. J Biol Chem. 274: 14229-37. PMID: 10318843
- Role of the Src homology 2 domains and interdomain regions in ZAP-70 phosphorylation and enzymatic activity. | Magistrelli, G., et al. 1999. Eur J Biochem. 266: 1166-73. PMID: 10583414
- Activation of Zap-70 tyrosine kinase due to a structural rearrangement induced by tyrosine phosphorylation and/or ITAM binding. | Visco, C., et al. 2000. Biochemistry. 39: 2784-91. PMID: 10704231
- The structure and signalling functions of the invariant T cell receptor components. | Frank, SJ., et al. 1990. Semin Immunol. 2: 89-97. PMID: 2151801
- The T cell receptor/CD3 complex: a dynamic protein ensemble. | Clevers, H., et al. 1988. Annu Rev Immunol. 6: 629-62. PMID: 3289580
- Identification by electrospray ionization mass spectrometry of the sites of tyrosine phosphorylation induced in activated Jurkat T cells on the protein tyrosine kinase ZAP-70. | Watts, JD., et al. 1994. J Biol Chem. 269: 29520-9. PMID: 7961936
- Enhancement of lymphocyte responsiveness by a gain-of-function mutation of ZAP-70. | Zhao, Q. and Weiss, A. 1996. Mol Cell Biol. 16: 6765-74. PMID: 8943331
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-ZAP-70 Antibody (pY319.17A) | sc-136248 | 200 µg/ml | $316.00 | |||
p-ZAP-70 Antibody (pY319.17A): m-IgGκ BP-HRP Bundle | sc-521346 | 200 µg Ab, 40 µg BP | $354.00 |