p-Smad3 Antibody (1D9): sc-517575

p-Smad3 Antibody (1D9) is a mouse monoclonal IgG antibody that detects Ser 425 phosphorylated Smad3 in mouse, rat, and human samples through immunofluorescence (IF) and immunohistochemistry with paraffin-embedded sections (IHCP). p-Smad3 (1D9) antibody is available in a non-conjugated form, allowing for versatile applications in research. Smad3 plays a crucial role in the transforming growth factor-beta (TGF-β) signaling pathway, which regulates various cellular processes, including cell growth, differentiation, and apoptosis. Phosphorylation of Smad3 at Ser 425 enhances transcriptional activity, influencing gene expression in response to TGF-β signals. This modification enables Smad3 to form complexes with Smad4 and translocate to the nucleus, where Smad3 regulates target gene transcription. Understanding Smad3 phosphorylation dynamics and implications in cellular signaling can provide insights into various pathological conditions, including cancer and fibrosis, making anti-p-Smad3 antibody (1D9) an invaluable tool for researchers studying these critical biological processes.

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p-Smad3 Antibody (1D9) References:

1. Global analysis of Smad2/3-dependent TGF-beta signaling in living mice reveals prominent tissue-specific responses to injury.  |  Lin, AH., et al. 2005. J Immunol. 175: 547-54. PMID: 15972691
2. Smads and chromatin modulation.  |  van Grunsven, LA., et al. 2005. Cytokine Growth Factor Rev. 16: 495-512. PMID: 15979924
3. TGF-beta signalling through the Smad pathway.  |  Massaous, J. and Hata, A. 1997. Trends Cell Biol. 7: 187-92. PMID: 17708943
4. A human Mad protein acting as a BMP-regulated transcriptional activator.  |  Liu, F., et al. 1996. Nature. 381: 620-3. PMID: 8637600
5. MADR2 maps to 18q21 and encodes a TGFbeta-regulated MAD-related protein that is functionally mutated in colorectal carcinoma.  |  Eppert, K., et al. 1996. Cell. 86: 543-52. PMID: 8752209
6. Receptor-associated Mad homologues synergize as effectors of the TGF-beta response.  |  Zhang, Y., et al. 1996. Nature. 383: 168-72. PMID: 8774881
7. Partnership between DPC4 and SMAD proteins in TGF-beta signalling pathways.  |  Lagna, G., et al. 1996. Nature. 383: 832-6. PMID: 8893010
8. Smad6 inhibits signalling by the TGF-beta superfamily.  |  Imamura, T., et al. 1997. Nature. 389: 622-6. PMID: 9335505
9. Smad8 mediates the signaling of the ALK-2 [corrected] receptor serine kinase.  |  Chen, Y., et al. 1997. Proc Natl Acad Sci U S A. 94: 12938-43. PMID: 9371779
10. TGF-beta signalling from cell membrane to nucleus through SMAD proteins.  |  Heldin, CH., et al. 1997. Nature. 390: 465-71. PMID: 9393997

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Smad3 Antibody (1D9) has 33 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Smad3 Antibody (1D9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Smad3 Antibody (1D9) Specifications

Alternate Product Name: Smad3

Product Clone Name: 1D9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG

Amino Acids: Ser 425

Epitope Target: Smad3

Epitope Species: human

Product Reactivity/Detection: Ser 425 phosphorylated Smad3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, IHC(P)

Vialing Information: Each vial contains 100 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, 1% glycerol and < 0.1% stabilizer protein

Research Citations: 33 citations in various scientific publications

Available Conjugates/Forms for p-Smad3 Antibody (1D9): N/A

The preferred Smad3 antibody is Smad3 Antibody (38-Q): sc-101154.