p-Rb Antibody (C-3): sc-377524
- p-Rb Antibody (C-3) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- recommended for detection of Thr 356 phosphorylated Rb of human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-Rb Antibody (C-3) for WB and IHC(P) applications. These reagents are now offered in bundles with p-Rb Antibody (C-3) (see ordering information below).
QUICK LINKS
SEE ALSO...
p-Rb Antibody (C-3) is a mouse monoclonal IgG2b antibody that detects retinoblastoma protein (Rb) in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Rb antibody (C-3) plays a crucial role in studying Rb, which is essential for regulating cell cycle progression, differentiation, and apoptosis. Rb protein is primarily located in the nucleus, where Rb functions as a key tumor suppressor by controlling the transition from the G1 phase to the S phase of the cell cycle. Proper localization of Rb is vital, as Rb interacts with transcription factors and other regulatory proteins that govern cell proliferation. Dysregulation of Rb localization can lead to uncontrolled cell division and contribute to tumorigenesis, particularly in pediatric cancers such as retinoblastoma. The phosphorylation state of Rb, influenced by various kinases, determines Rb activity and localization, making Rb a critical focus for understanding cancer biology and developing targeted therapies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p-Rb Antibody (C-3) References:
- Biochemical properties of the stimulatory activity of DNA polymerase alpha by the hyper-phosphorylated retinoblastoma protein. | Takemura, M. 2002. Biochim Biophys Acta. 1571: 151-6. PMID: 12049795
- Phosphorylated retinoblastoma protein complexes with pp32 and inhibits pp32-mediated apoptosis. | Adegbola, O. and Pasternack, GR. 2005. J Biol Chem. 280: 15497-502. PMID: 15716273
- Hyper-phosphorylated retinoblastoma protein suppresses telomere elongation. | Takemura, M., et al. 2008. Biosci Biotechnol Biochem. 72: 630-5. PMID: 18256459
- Discrete phosphorylated retinoblastoma protein isoform expression in mouse tooth development. | Zhang, W., et al. 2012. J Mol Histol. 43: 281-8. PMID: 22476877
- Phosphorylated retinoblastoma protein (p-Rb) is involved in neuronal apoptosis after traumatic brain injury in adult rats. | Liu, W., et al. 2013. J Mol Histol. 44: 147-58. PMID: 23371354
- Phosphorylated retinoblastoma protein is a potential predictive marker of irinotecan efficacy for colorectal cancer. | Ikai, A., et al. 2016. Int J Oncol. 48: 1297-304. PMID: 26783196
- CDK4/6 inhibitor-SHR6390 exerts potent antitumor activity in esophageal squamous cell carcinoma by inhibiting phosphorylated Rb and inducing G1 cell cycle arrest. | Wang, J., et al. 2017. J Transl Med. 15: 127. PMID: 28578693
- Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression. | Jin, X., et al. 2019. Mol Cell. 73: 22-35.e6. PMID: 30527665
- SETDB1 Modulates Degradation of Phosphorylated RB and Anticancer Efficacy of CDK4/6 Inhibitors. | Huang, Z., et al. 2023. Cancer Res. 83: 875-889. PMID: 36637424
- Discovery of Novel Antitumor Small-Molecule Agent with Dual Action of CDK2/p-RB and MDM2/p53. | Liu, Z., et al. 2024. Molecules. 29: PMID: 38338471
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Rb Antibody (C-3) | sc-377524 | 200 µg/ml | $322.00 | |||
p-Rb Antibody (C-3): m-IgG Fc BP-HRP Bundle | sc-538050 | 200 µg Ab; 10 µg BP | $361.00 | |||
p-Rb Antibody (C-3): m-IgGκ BP-HRP Bundle | sc-535576 | 200 µg Ab; 40 µg BP | $361.00 | |||
p-Rb Antibody (C-3): m-IgG2b BP-HRP Bundle | sc-549915 | 200 µg Ab; 10 µg BP | $361.00 | |||
p-Rb (C-3) Neutralizing Peptide | sc-377524 P | 100 µg/0.5 ml | $69.00 |