p-NF-M Antibody (RNF406): sc-32727

p-NF-M Antibody (RNF406) is a mouse monoclonal IgG1 light chain antibody that detects neurofilament-M (NF-M) in mouse, rat, human, Xenopus, and bovine samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-NF-M antibody (RNF406) specifically recognizes phosphorylated forms of NF-M, which is a crucial component of the neuronal cytoskeleton and plays a significant role in maintaining axon structural integrity. NF-M phosphorylation regulates neurofilament assembly and stability, influencing axon caliber and neuronal function. Dysregulation of NF-M phosphorylation has been implicated in various neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson′s disease, and Alzheimer′s disease, highlighting NF-M′s relevance in both normal physiology and disease states. NF-M monoclonal antibody (RNF406) enables researchers to gain valuable insights into neurofilament phosphorylation dynamics and their impact on neuronal health and disease progression.

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p-NF-M Antibody (RNF406) References:

1. Activation of mitogen-activated protein kinases (Erk1 and Erk2) cascade results in phosphorylation of NF-M tail domains in transfected NIH 3T3 cells.  |  Li, BS., et al. 1999. Eur J Biochem. 262: 211-7. PMID: 10231383
2. Monoclonal antibody NF-09 specific for neurofilament protein NF-M.  |  Dráberová, E., et al. 1999. Folia Biol (Praha). 45: 163-5. PMID: 10732731
3. Phosphorylation of the head domain of neurofilament protein (NF-M): a factor regulating topographic phosphorylation of NF-M tail domain KSP sites in neurons.  |  Zheng, YL., et al. 2003. J Biol Chem. 278: 24026-32. PMID: 12695506
4. NF-M is an essential target for the myelin-directed 'outside-in' signaling cascade that mediates radial axonal growth.  |  Garcia, ML., et al. 2003. J Cell Biol. 163: 1011-20. PMID: 14662745
5. Regulation between O-GlcNAcylation and phosphorylation of neurofilament-M and their dysregulation in Alzheimer disease.  |  Deng, Y., et al. 2008. FASEB J. 22: 138-45. PMID: 17687114
6. Phosphorylation of neurofilament subunit NF-M is regulated by activation of NMDA receptors and modulates cytoskeleton stability and neuronal shape.  |  Fiumelli, H., et al. 2008. Cell Motil Cytoskeleton. 65: 495-504. PMID: 18412220
7. The C-terminal domains of NF-H and NF-M subunits maintain axonal neurofilament content by blocking turnover of the stationary neurofilament network.  |  Rao, MV., et al. 2012. PLoS One. 7: e44320. PMID: 23028520
8. Complete amino acid sequence and in vitro expression of rat NF-M, the middle molecular weight neurofilament protein.  |  Napolitano, EW., et al. 1987. J Neurosci. 7: 2590-9. PMID: 2441012
9. Phosphorylation of native and reassembled neurofilaments composed of NF-L, NF-M, and NF-H by the catalytic subunit of cAMP-dependent protein kinase.  |  Hisanaga, S., et al. 1994. Mol Biol Cell. 5: 161-72. PMID: 8019002
10. A monoclonal antibody recognizing nestin and the phosphorylated form of neurofilament-M.  |  Sakuma, H., et al. 1997. Dev Neurosci. 19: 348-56. PMID: 9215881

The preferred NF-M antibody is NF-M Antibody (E-9): sc-398532.