p-NF-M Antibody (RNF403): sc-32726

p-NF-M Antibody (RNF403) is a mouse monoclonal IgG1 antibody that detects neurofilament-M (NF-M) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-NF-M antibody (RNF403) specifically recognizes phosphorylated forms of NF-M, which is a crucial component of the neuronal cytoskeleton and plays a significant role in maintaining the structural integrity of axons. NF-M phosphorylation regulates the assembly and stability of neurofilaments, influencing axon caliber and neuronal signaling. Dysregulation of NF-M phosphorylation has been implicated in various neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS), Parkinson′s disease, and Alzheimer′s disease, highlighting NF-M monoclonal antibody (RNF403)′s potential utility in research focused on these conditions. By providing insights into the phosphorylation status of NF-M, NF-M (RNF403) antibody can aid in understanding the underlying mechanisms of neurodegeneration and the role of neurofilaments in neuronal health and disease.

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p-NF-M Antibody (RNF403) References:

1. Casein kinase II is responsible for phosphorylation of NF-L at Ser-473.  |  Nakamura, Y., et al. 1999. FEBS Lett. 455: 83-6. PMID: 10428477
2. Neurofilament metabolism in sporadic amyotrophic lateral sclerosis.  |  Strong, MJ. 1999. J Neurol Sci. 169: 170-7. PMID: 10540027
3. Metal ion-induced conformational changes of phosphorylated fragments of human neurofilament (NF-M) protein.  |  Hollósi, M., et al. 1992. J Mol Biol. 223: 673-82. PMID: 1542114
4. Effects of phosphorylation of the neurofilament L protein on filamentous structures.  |  Hisanaga, S., et al. 1990. Cell Regul. 1: 237-48. PMID: 2100199
5. Involvement of protein kinase C in the regulation of assembly-disassembly of neurofilaments in vitro.  |  Gonda, Y., et al. 1990. Biochem Biophys Res Commun. 167: 1316-25. PMID: 2108674
6. In vivo phosphorylation of distinct domains of the 70-kilodalton neurofilament subunit involves different protein kinases.  |  Sihag, RK. and Nixon, RA. 1989. J Biol Chem. 264: 457-64. PMID: 2491851
7. Assembly and exchange of intermediate filament proteins of neurons: neurofilaments are dynamic structures.  |  Angelides, KJ., et al. 1989. J Cell Biol. 108: 1495-506. PMID: 2925792
8. Structure and evolutionary origin of the gene encoding mouse NF-M, the middle-molecular-mass neurofilament protein.  |  Levy, E., et al. 1987. Eur J Biochem. 166: 71-7. PMID: 3036526
9. Monoclonal antibodies distinguish several differentially phosphorylated states of the two largest rat neurofilament subunits (NF-H and NF-M) and demonstrate their existence in the normal nervous system of adult rats.  |  Lee, VM., et al. 1987. J Neurosci. 7: 3474-88. PMID: 3119789
10. Increasing neurofilament subunit NF-M expression reduces axonal NF-H, inhibits radial growth, and results in neurofilamentous accumulation in motor neurons.  |  Wong, PC., et al. 1995. J Cell Biol. 130: 1413-22. PMID: 7559762
11. Abnormal distribution of neurofilament L in neurons with Alzheimer's disease.  |  Nakamura, Y., et al. 1997. Neurosci Lett. 225: 201-4. PMID: 9147405
12. Mitogen-activated protein kinases (Erk1,2) phosphorylate Lys-Ser-Pro (KSP) repeats in neurofilament proteins NF-H and NF-M.  |  Veeranna,., et al. 1998. J Neurosci. 18: 4008-21. PMID: 9592082

The preferred NF-M antibody is NF-M Antibody (E-9): sc-398532.