p-MAPKAPK-2 Antibody (83.Thr 334): sc-293139

p-MAPKAPK-2 Antibody (83.Thr 334) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Thr 334 phosphorylated MAPKAPK-2 protein of human origin by western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). anti-p-MAPKAPK-2 antibody (83.Thr 334) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The p38 mitogen-activated protein kinase (MAPK) pathway plays a crucial role in mediating cellular responses to environmental stress, and MAPKAP kinases, including MAPKAPK-2, are key substrates in this signaling cascade. Upon activation, p38 phosphorylates MAPKAPK-2, which in turn phosphorylates several important nuclear targets such as CREB/ATF1 and serum response factor, as well as cytoplasmic targets like HSP25/27 and glycogen synthase. Phosphorylation of threonine 334, strategically located between the kinase domain and C-terminal regulatory domain, acts as a regulatory switch for nuclear import and export of MAPKAPK-2. This modification influences protein localization and functional interactions, as phosphorylated MAPKAPK-2 can mask nuclear localization signal while exposing nuclear export signal, facilitating p38 transport to cytoplasm. Understanding these phosphorylation dynamics is essential for elucidating broader implications of MAPKAPK-2 in stress response and cellular signaling pathways.

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p-MAPKAPK-2 Antibody (83.Thr 334) References:

1. p38 Kinase-dependent MAPKAPK-2 activation functions as 3-phosphoinositide-dependent kinase-2 for Akt in human neutrophils.  |  Rane, MJ., et al. 2001. J Biol Chem. 276: 3517-23. PMID: 11042204
2. Structure of mitogen-activated protein kinase-activated protein (MAPKAP) kinase 2 suggests a bifunctional switch that couples kinase activation with nuclear export.  |  Meng, W., et al. 2002. J Biol Chem. 277: 37401-5. PMID: 12171911
3. Both binding and activation of p38 mitogen-activated protein kinase (MAPK) play essential roles in regulation of the nucleocytoplasmic distribution of MAPK-activated protein kinase 5 by cellular stress.  |  Seternes, OM., et al. 2002. Mol Cell Biol. 22: 6931-45. PMID: 12242275
4. MAPKAP kinase-2; a novel protein kinase activated by mitogen-activated protein kinase.  |  Stokoe, D., et al. 1992. EMBO J. 11: 3985-94. PMID: 1327754
5. In vivo and in vitro SAR of tetracyclic MAPKAP-K2 (MK2) inhibitors. Part II.  |  Revesz, L., et al. 2010. Bioorg Med Chem Lett. 20: 4719-23. PMID: 20591669
6. Phosphorylation of cAMP-specific PDE4A5 (phosphodiesterase-4A5) by MK2 (MAPKAPK2) attenuates its activation through protein kinase A phosphorylation.  |  MacKenzie, KF., et al. 2011. Biochem J. 435: 755-69. PMID: 21323643
7. γ-secretase inhibitor DAPT sensitizes t-AUCB-induced apoptosis of human glioblastoma cells in vitro via blocking the p38 MAPK/MAPKAPK2/Hsp27 pathway.  |  Li, JY., et al. 2014. Acta Pharmacol Sin. 35: 825-31. PMID: 24793313
8. mTOR regulates MAPKAPK2 translation to control the senescence-associated secretory phenotype.  |  Herranz, N., et al. 2015. Nat Cell Biol. 17: 1205-17. PMID: 26280535
9. Insulin-stimulated MAP-2 kinase phosphorylates and activates ribosomal protein S6 kinase II.  |  Sturgill, TW., et al. 1988. Nature. 334: 715-8. PMID: 2842685
10. MAPKAPK2 (MK2) inhibition mediates radiation-induced inflammatory cytokine production and tumor growth in head and neck squamous cell carcinoma.  |  Berggren, KL., et al. 2019. Oncogene. 38: 7329-7341. PMID: 31417185
11. RSK3 encodes a novel pp90rsk isoform with a unique N-terminal sequence: growth factor-stimulated kinase function and nuclear translocation.  |  Zhao, Y., et al. 1995. Mol Cell Biol. 15: 4353-63. PMID: 7623830
12. The mitogen-activated protein kinase signal transduction pathway.  |  Davis, RJ. 1993. J Biol Chem. 268: 14553-6. PMID: 8325833

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-MAPKAPK-2 Antibody (83.Thr 334) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-p-MAPKAPK-2 Antibody (83.Thr 334) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-MAPKAPK-2 Antibody (83.Thr 334) Specifications

Alternate Product Name: MAPKAPK-2

Product Clone Name: 83.Thr 334

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Thr 334

Epitope Target: MAPKAPK-2

Epitope Species: human

Product Reactivity/Detection: Thr 334 phosphorylated MAPKAPK-2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for p-MAPKAPK-2 Antibody (83.Thr 334): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MAPKAPK-2 antibody is MAPKAPK-2 Antibody (A-11): sc-393609.