p-LKB1 Antibody (C-1): sc-271924

p-LKB1 Antibody (C-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects Ser 431 phosphorylated LKB1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-LKB1 (C-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. LKB1, also known as STK11, is a critical serine/threonine kinase composed of 433 amino acids, playing a vital role in cellular energy homeostasis and tumor suppression. LKB1 is predominantly located in the cytoplasm and is essential for regulating cell growth and metabolism by activating AMP-activated protein kinase (AMPK) in response to cellular stress. Proper localization of LKB1 ensures phosphorylation of downstream targets that mediate energy balance and cell cycle control. Mutations in LKB1 are associated with Peutz-Jeghers Syndrome, a hereditary condition that increases the risk of various cancers, highlighting LKB1′s importance in maintaining cellular integrity and preventing tumorigenesis. Phosphorylation at Ser431 by kinases such as p90 ribosomal S6 kinase and cAMP-dependent protein kinase is necessary for LKB1′s function in suppressing cell growth, making anti-p-LKB1 antibody (C-1) an invaluable tool for studying LKB1′s role in cancer biology and metabolic regulation.

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p-LKB1 Antibody (C-1) References:

1. Phosphorylation of the protein kinase mutated in Peutz-Jeghers cancer syndrome, LKB1/STK11, at Ser431 by p90(RSK) and cAMP-dependent protein kinase, but not its farnesylation at Cys(433), is essential for LKB1 to suppress cell vrowth.  |  Sapkota, GP., et al. 2001. J Biol Chem. 276: 19469-82. PMID: 11297520
2. C-terminal phosphorylation of LKB1 is not required for regulation of AMP-activated protein kinase, BRSK1, BRSK2, or cell cycle arrest.  |  Fogarty, S. and Hardie, DG. 2009. J Biol Chem. 284: 77-84. PMID: 18854318
3. Improved myocardial perfusion in chronic diabetic mice by the up-regulation of pLKB1 and AMPK signaling.  |  Kusmic, C., et al. 2010. J Cell Biochem. 109: 1033-44. PMID: 20108250
4. Phosphorylation of serine 399 in LKB1 protein short form by protein kinase Cζ is required for its nucleocytoplasmic transport and consequent AMP-activated protein kinase (AMPK) activation.  |  Zhu, H., et al. 2013. J Biol Chem. 288: 16495-16505. PMID: 23612973
5. Peutz-Jeghers syndrome is caused by mutations in a novel serine threonine kinase.  |  Jenne, DE., et al. 1998. Nat Genet. 18: 38-43. PMID: 9425897
6. A serine/threonine kinase gene defective in Peutz-Jeghers syndrome.  |  Hemminki, A., et al. 1998. Nature. 391: 184-7. PMID: 9428765
7. Low frequency of somatic mutations in the LKB1/Peutz-Jeghers syndrome gene in sporadic breast cancer.  |  Bignell, GR., et al. 1998. Cancer Res. 58: 1384-6. PMID: 9537235
8. Somatic mutations in LKB1 are rare in sporadic colorectal and testicular tumors.  |  Avizienyte, E., et al. 1998. Cancer Res. 58: 2087-90. PMID: 9605748
9. STK11 mutations in Peutz-Jeghers syndrome and sporadic colon cancer.  |  Resta, N., et al. 1998. Cancer Res. 58: 4799-801. PMID: 9809980
10. Loss of LKB1 kinase activity in Peutz-Jeghers syndrome, and evidence for allelic and locus heterogeneity.  |  Mehenni, H., et al. 1998. Am J Hum Genet. 63: 1641-50. PMID: 9837816

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-LKB1 Antibody (C-1) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-p-LKB1 Antibody (C-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-LKB1 Antibody (C-1) Specifications

Alternate Product Name: STK11

Product Clone Name: C-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: Ser 431

Epitope Target: LKB-1

Epitope Species: mouse

Product Reactivity/Detection: Ser 431 phosphorylated LKB1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for p-LKB1 Antibody (C-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred LKB1 antibody is LKB1 Antibody (Ley 37D/G6): sc-32245.