Nrdp1 Antibody (B-8): sc-374120

Nrdp1 Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nrdp1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Nrdp1 antibody (B-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nrdp1, also known as RNF41, SBBI03, or FLRF, is a 317 amino acid protein characterized by the presence of one RING-type zinc finger and one SIAH-type zinc finger, which are crucial for function as an E3 ubiquitin-protein ligase. Nrdp1 plays a vital role in the ubiquitination pathway, facilitating the degradation of specific substrates by transferring ubiquitin from an E2 ubiquitin-conjugating enzyme to target proteins. Nrdp1 (B-8) monoclonal antibody interacts with ErbB-3 and UBPY, marking them for proteasomal degradation, which is essential for regulating cellular signaling pathways and maintaining protein homeostasis. Nrdp1 expression in tissues such as the testis, ovary, and prostate highlights importance in reproductive biology and cellular regulation.

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Nrdp1 Antibody (B-8) References:

1. FLRF, a novel evolutionarily conserved RING finger gene, is differentially expressed in mouse fetal and adult hematopoietic stem cells and progenitors.  |  Abdullah, JM., et al. 2001. Blood Cells Mol Dis. 27: 320-33. PMID: 11358394
2. An RBCC protein implicated in maintenance of steady-state neuregulin receptor levels.  |  Diamonti, AJ., et al. 2002. Proc Natl Acad Sci U S A. 99: 2866-71. PMID: 11867753
3. Nrdp1/FLRF is a ubiquitin ligase promoting ubiquitination and degradation of the epidermal growth factor receptor family member, ErbB3.  |  Qiu, XB. and Goldberg, AL. 2002. Proc Natl Acad Sci U S A. 99: 14843-8. PMID: 12411582
4. Nrdp1-mediated degradation of the gigantic IAP, BRUCE, is a novel pathway for triggering apoptosis.  |  Qiu, XB., et al. 2004. EMBO J. 23: 800-10. PMID: 14765125
5. Stabilization of the E3 ubiquitin ligase Nrdp1 by the deubiquitinating enzyme USP8.  |  Wu, X., et al. 2004. Mol Cell Biol. 24: 7748-57. PMID: 15314180
6. Amino-terminal dimerization, NRDP1-rhodanese interaction, and inhibited catalytic domain conformation of the ubiquitin-specific protease 8 (USP8).  |  Avvakumov, GV., et al. 2006. J Biol Chem. 281: 38061-70. PMID: 17035239
7. Neuregulin-induced ErbB3 downregulation is mediated by a protein stability cascade involving the E3 ubiquitin ligase Nrdp1.  |  Cao, Z., et al. 2007. Mol Cell Biol. 27: 2180-8. PMID: 17210635
8. Structure-based mutagenesis of the substrate-recognition domain of Nrdp1/FLRF identifies the binding site for the receptor tyrosine kinase ErbB3.  |  Bouyain, S. and Leahy, DJ. 2007. Protein Sci. 16: 654-61. PMID: 17384230
9. New insights into the role of the ubiquitin-proteasome pathway in the regulation of apoptosis.  |  Liu, CH., et al. 2007. Chang Gung Med J. 30: 469-79. PMID: 18350730
10. Overexpression of Nrdp1/FLRF sensitizes cells to oxidative stress.  |  Zhou, A., et al. 2011. Biochem Biophys Res Commun. 410: 771-4. PMID: 21693106
11. Nrdp1 expression to predict clinical outcome and efficacy of adjuvant anthracyclines-based chemotherapy in breast cancer: A retrospective study.  |  Jiao, S., et al. 2015. Cancer Biomark. 15: 115-23. PMID: 25519010
12. A20/Nrdp1 interaction alters the inflammatory signaling profile by mediating K48- and K63-linked polyubiquitination of effectors MyD88 and TBK1.  |  Meng, Z., et al. 2021. J Biol Chem. 297: 100811. PMID: 34023381

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nrdp1 Antibody (B-8) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Nrdp1 Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nrdp1 Antibody (B-8) Specifications

Alternate Product Name: Nrdp1

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-317

Epitope Target: Nrdp1

Epitope Species: human

Product Reactivity/Detection: Nrdp1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Nrdp1 Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Nrdp1 antibody is Nrdp1 Antibody (A-6): sc-365622.