NHE-8 Antibody (7A11): sc-53902
- NHE-8 Antibody (7A11) is a mouse monoclonal IgG1 κ NHE-8 antibody provided at 200 µg/ml
- raised against an MBP fusion protein containing the C-terminal 89 amino acids of NHE-8 of human origin
- NHE-8 Antibody (7A11) is recommended for detection of NHE-8 of mouse, rat and human origin by WB, IP, IF and IHC(P)
- Anti-NHE-8 Antibody (7A11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NHE-8 Antibody (7A11) for WB and IHC(P) applications. These reagents are now offered in bundles with NHE-8 Antibody (7A11) (see ordering information below).
NHE-8 Antibody (7A11) is a mouse monoclonal IgG1 kappa light chain antibody that detects NHE-8 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). NHE-8 (7A11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NHE-8 protein, consisting of 575 amino acids, is primarily localized apically in intestinal epithelial cells, where NHE-8 plays a crucial role in regulating sodium ion absorption. This localization allows NHE-8 to effectively mediate the exchange of sodium (Na+) and hydrogen (H+) ions, which is essential for maintaining electrolyte balance and pH homeostasis in the intestinal environment. NHE-8 expression is higher in young mammals compared to adults, indicating a vital role during early development when efficient nutrient absorption is critical. NHE-8 gene and protein expression remains tightly regulated throughout ontogeny, suggesting particular importance for intestinal function in early stages of mammalian life.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NHE-8 Antibody (7A11) References:
- Cloning and expression of a chloride-dependent Na+-H+ exchanger. | Sangan, P., et al. 2002. J Biol Chem. 277: 9668-75. PMID: 11773056
- Renal expression of novel Na+/H+ exchanger isoform NHE8. | Goyal, S., et al. 2003. Am J Physiol Renal Physiol. 284: F467-73. PMID: 12409279
- Immunolocalization of NHE8 in rat kidney. | Goyal, S., et al. 2005. Am J Physiol Renal Physiol. 288: F530-8. PMID: 15522984
- Subcloning, localization, and expression of the rat intestinal sodium-hydrogen exchanger isoform 8. | Xu, H., et al. 2005. Am J Physiol Gastrointest Liver Physiol. 289: G36-41. PMID: 15731506
- Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally related proteins. | Orlowski, J., et al. 1992. J Biol Chem. 267: 9331-9. PMID: 1577762
- Epithelial localization of a reptilian Na+/H+ exchanger homologous to NHE-1. | Harris, SP., et al. 1997. Am J Physiol. 272: G1594-606. PMID: 9227498
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NHE-8 Antibody (7A11) | sc-53902 | 200 µg/ml | $316.00 | |||
NHE-8 Antibody (7A11): m-IgG Fc BP-HRP Bundle | sc-528586 | 200 µg Ab; 10 µg BP | $354.00 | |||
NHE-8 Antibody (7A11): m-IgGκ BP-HRP Bundle | sc-520998 | 200 µg Ab, 40 µg BP | $354.00 | |||
NHE-8 Antibody (7A11): m-IgG1 BP-HRP Bundle | sc-543010 | 200 µg Ab; 20 µg BP | $354.00 | |||
NHE-8 Antibody (7A11) AC | sc-53902 AC | 500 µg/ml, 25% agarose | $416.00 | |||
NHE-8 Antibody (7A11) HRP | sc-53902 HRP | 200 µg/ml | $316.00 | |||
NHE-8 Antibody (7A11) FITC | sc-53902 FITC | 200 µg/ml | $330.00 | |||
NHE-8 Antibody (7A11) PE | sc-53902 PE | 200 µg/ml | $343.00 | |||
NHE-8 Antibody (7A11) Alexa Fluor® 488 | sc-53902 AF488 | 200 µg/ml | $357.00 | |||
NHE-8 Antibody (7A11) Alexa Fluor® 546 | sc-53902 AF546 | 200 µg/ml | $357.00 | |||
NHE-8 Antibody (7A11) Alexa Fluor® 594 | sc-53902 AF594 | 200 µg/ml | $357.00 | |||
NHE-8 Antibody (7A11) Alexa Fluor® 647 | sc-53902 AF647 | 200 µg/ml | $357.00 | |||
NHE-8 Antibody (7A11) Alexa Fluor® 680 | sc-53902 AF680 | 200 µg/ml | $357.00 | |||
NHE-8 Antibody (7A11) Alexa Fluor® 790 | sc-53902 AF790 | 200 µg/ml | $357.00 |