NHE-3 Antibody (4F5): sc-53963

NHE-3 Antibody (4F5) is a mouse monoclonal IgG1 antibody that detects NHE-3 in mouse, rat, and rabbit samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. NHE-3, a member of the Na+/H+ exchanger family, plays a crucial role in maintaining cellular homeostasis by regulating sodium and hydrogen ion transport across the plasma membrane. NHE-3 is predominantly located in the apical membrane of renal proximal tubules, where NHE-3 is essential for sodium reabsorption and fluid balance. Proper functioning of NHE-3 is vital for the kidneys to manage blood pressure and fluid volume, as NHE-3 facilitates the reabsorption of sodium ions while excreting hydrogen ions, thus influencing overall electrolyte balance. Additionally, NHE-3 is expressed in the gastric mucosa, where NHE-3 protects the stomach lining by secreting protons that help maintain the acidic environment necessary for digestion. Anti-NHE-3 antibody (4F5) is an invaluable tool for researchers studying renal physiology, electrolyte transport, and related pathophysiological conditions, providing insights into the mechanisms underlying sodium homeostasis and its implications in health and disease.

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NHE-3 Antibody (4F5) References:

1. cAMP-induced phosphorylation and inhibition of Na(+)/H(+) exchanger 3 (NHE3) are dependent on the presence but not the phosphorylation of NHE regulatory factor.  |  Zizak, M., et al. 1999. J Biol Chem. 274: 24753-8. PMID: 10455146
2. Expression and cell-specific and membrane-specific localization of NHE-3 in the human and guinea pig upper gastrointestinal tract.  |  Kulaksiz, H., et al. 2001. Cell Tissue Res. 303: 337-43. PMID: 11320649
3. Cloning and expression of a chloride-dependent Na+-H+ exchanger.  |  Sangan, P., et al. 2002. J Biol Chem. 277: 9668-75. PMID: 11773056
4. Na+/H+ exchange activity and NHE-3 expression in renal tubules from the spontaneously hypertensive rat.  |  LaPointe, MS., et al. 2002. Kidney Int. 62: 157-65. PMID: 12081574
5. Renal expression of novel Na+/H+ exchanger isoform NHE8.  |  Goyal, S., et al. 2003. Am J Physiol Renal Physiol. 284: F467-73. PMID: 12409279
6. Immunolocalization of NHE8 in rat kidney.  |  Goyal, S., et al. 2005. Am J Physiol Renal Physiol. 288: F530-8. PMID: 15522984
7. Subcloning, localization, and expression of the rat intestinal sodium-hydrogen exchanger isoform 8.  |  Xu, H., et al. 2005. Am J Physiol Gastrointest Liver Physiol. 289: G36-41. PMID: 15731506
8. Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally related proteins.  |  Orlowski, J., et al. 1992. J Biol Chem. 267: 9331-9. PMID: 1577762
9. Dexamethasone increases fluid absorption via Na+/H+ exchanger (NHE) 3 activation in normal human middle ear epithelial cells.  |  Choi, JY., et al. 2006. Eur J Pharmacol. 536: 12-8. PMID: 16564041
10. Renal NHE expression and activity in neonatal NHE3- and NHE8-null mice.  |  Pirojsakul, K., et al. 2015. Am J Physiol Renal Physiol. 308: F31-8. PMID: 25377912
11. Downregulation of NHE-3 (SLC9A3) expression by MicroRNAs in intestinal epithelial cells.  |  Anbazhagan, AN., et al. 2022. Am J Physiol Cell Physiol. 323: C1720-C1727. PMID: 36189974
12. Epithelial localization of a reptilian Na+/H+ exchanger homologous to NHE-1.  |  Harris, SP., et al. 1997. Am J Physiol. 272: G1594-606. PMID: 9227498

The preferred p-NHE-3 antibody is p-NHE-3 Antibody (14D5): sc-53962.