NF-E2 Antibody (G-2): sc-514429

NF-E2 Antibody (G-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects NF-E2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NF-E2 (G-2) antibody is available as the non-conjugated form. NF-E2 protein plays a crucial role in regulating globulin gene expression in developing erythroid cells, vital for proper red blood cell formation and function. This regulation occurs through NF-E2′s interaction with specific upstream AP-1-like recognition sites, particularly a site containing an intact AP-1 binding motif, with a G residue located two base pairs upstream. NF-E2 is an obligate heterodimer composed of two subunits: NF-E2 p45 and NF-E2 p18. NF-E2 p18, also known as NF-E2U or MAFK, is a ubiquitously expressed component related to the v-Maf oncogene and features a basic-leucine zipper domain essential for DNA binding and dimerization. NF-E2 p18 may contribute to erythroid differentiation, highlighting its importance in hematopoiesis. NF-E2 p45, the major component, belongs to the basic region-leucine zipper family of transcription factors and is specifically expressed at low levels in hematopoietic progenitor cells and differentiated cells of the erythroid, megakaryocyte, and mast cell lineages, underscoring its significance in blood cell development.

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NF-E2 Antibody (G-2) References:

1. NF-E2-related factor 2 and its role in diabetic nephropathy.  |  Dadras, F. and Khoshjou, F. 2013. Iran J Kidney Dis. 7: 346-51. PMID: 24072144
2. Regulation and function of the NFE2 transcription factor in hematopoietic and non-hematopoietic cells.  |  Gasiorek, JJ. and Blank, V. 2015. Cell Mol Life Sci. 72: 2323-35. PMID: 25721735
3. NF-E2, FLI1 and RUNX1 collaborate at areas of dynamic chromatin to activate transcription in mature mouse megakaryocytes.  |  Zang, C., et al. 2016. Sci Rep. 6: 30255. PMID: 27457419
4. p45 NF-E2 regulates syncytiotrophoblast differentiation by post-translational GCM1 modifications in human intrauterine growth restriction.  |  Kohli, S., et al. 2017. Cell Death Dis. 8: e2730. PMID: 28383551
5. Epigenetic regulation of NFE2 overexpression in myeloproliferative neoplasms.  |  Peeken, JC., et al. 2018. Blood. 131: 2065-2073. PMID: 29519804
6. Altered NFE2 activity predisposes to leukemic transformation and myelosarcoma with AML-specific aberrations.  |  Jutzi, JS., et al. 2019. Blood. 133: 1766-1777. PMID: 30755419
7. Nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) in non-small cell lung cancer.  |  Zeng, Z., et al. 2020. Life Sci. 254: 117325. PMID: 31954159
8. Nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2) in autophagy-induced hepatocellular carcinoma.  |  Zhou, J., et al. 2020. Clin Chim Acta. 506: 1-8. PMID: 32109431
9. Loss of NF-E2 expression contributes to the induction of profibrotic signaling in diabetic kidneys.  |  Jin, S., et al. 2020. Life Sci. 254: 117783. PMID: 32413404
10. The histone demethylase JMJD2C constitutes a novel NFE2 target gene that is required for the survival of JAK2V617F mutated cells.  |  Staehle, AM., et al. 2023. Leukemia. 37: 919-923. PMID: 36709354

The preferred NF-E2 antibody is NF-E2 Antibody (D-6): sc-365083.