NF-E2 Antibody (D-6): sc-365083

NF-E2 Antibody (D-6) is a mouse monoclonal IgM kappa antibody that detects NF-E2 p45 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NF-E2 (D-6) antibody is available in a non-conjugated form, allowing for versatile use in various experimental setups. NF-E2 p45 plays a crucial role as a nuclear DNA binding protein that regulates globulin gene expression during erythroid cell development, which is vital for proper red blood cell formation and function. NF-E2 p45 binds to a site that includes an intact AP-1 binding motif, essential for globin gene transcriptional activation. NF-E2 p45 functions as an obligate heterodimer with NF-E2 p18, a ubiquitously expressed component related to the v-Maf oncogene. NF-E2 p18 contains a basic-leucine zipper domain important for both DNA binding and dimerization, and may contribute to erythroid differentiation. NF-E2 p45, a member of the basic region-leucine zipper family of transcription factors, is expressed at low levels in hematopoietic progenitor cells as well as in differentiated erythroid, megakaryocyte, and mast cell lineages, highlighting its significance in hematopoiesis and cellular differentiation.

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NF-E2 Antibody (D-6) References:

1. Regulation of mouse p45 NF-E2 transcription by an erythroid-specific GATA-dependent intronic alternative promoter.  |  Moroni, E., et al. 2000. J Biol Chem. 275: 10567-76. PMID: 10744751
2. Activation of beta-major globin gene transcription is associated with recruitment of NF-E2 to the beta-globin LCR and gene promoter.  |  Sawado, T., et al. 2001. Proc Natl Acad Sci U S A. 98: 10226-31. PMID: 11517325
3. Characterization of the major regulatory element upstream of the human alpha-globin gene cluster.  |  Jarman, AP., et al. 1991. Mol Cell Biol. 11: 4679-89. PMID: 1875946
4. Tandem AP-1-binding sites within the human beta-globin dominant control region function as an inducible enhancer in erythroid cells.  |  Ney, PA., et al. 1990. Genes Dev. 4: 993-1006. PMID: 2116990
5. The beta-globin dominant control region: hypersensitive site 2.  |  Philipsen, S., et al. 1990. EMBO J. 9: 2159-67. PMID: 2357964
6. Two tissue-specific factors bind the erythroid promoter of the human porphobilinogen deaminase gene.  |  Mignotte, V., et al. 1989. Nucleic Acids Res. 17: 37-54. PMID: 2911469
7. Experimental Nonalcoholic Steatohepatitis and Liver Fibrosis Are Ameliorated by Pharmacologic Activation of Nrf2 (NF-E2 p45-Related Factor 2).  |  Sharma, RS., et al. 2018. Cell Mol Gastroenterol Hepatol. 5: 367-398. PMID: 29552625
8. Targeting the NF-E2-related factor 2 pathway for overcoming leukemia.  |  Harifi-Mood, MS., et al. 2023. Int J Biol Macromol. 253: 127594. PMID: 37890739
9. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.  |  Kataoka, K., et al. 1995. Mol Cell Biol. 15: 2180-90. PMID: 7891713
10. Regulation of transcription by dimerization of erythroid factor NF-E2 p45 with small Maf proteins.  |  Igarashi, K., et al. 1994. Nature. 367: 568-72. PMID: 8107826
11. Erythroid transcription factor NF-E2 is a haematopoietic-specific basic-leucine zipper protein.  |  Andrews, NC., et al. 1993. Nature. 362: 722-8. PMID: 8469283
12. Mouse microcytic anaemia caused by a defect in the gene encoding the globin enhancer-binding protein NF-E2.  |  Peters, LL., et al. 1993. Nature. 362: 768-70. PMID: 8469289

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NF-E2 Antibody (D-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-NF-E2 Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NF-E2 Antibody (D-6) Specifications

Alternate Product Name: NF-E2

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 343-369

Epitope Target: NF-E2

Epitope Species: mouse

Product Reactivity/Detection: NF-E2 p45

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for NF-E2 Antibody (D-6): P: Blocking Peptide, X: TransCruz Reagent