Neutrophil Elastase Antibody (F-1): sc-55548

Neutrophil Elastase Antibody (F-1) is a mouse monoclonal IgG1 kappa light chain antibody designed to recognize amino acids 211-267 of human Neutrophil Elastase (NE), also known as ELANE or medullasin. Anti-NE antibody (F-1) effectively detects NE through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications, making this tool versatile for various research settings. NE is a serine protease with a well-defined two-domain structure, comprising a conserved N-terminal domain that ensures enzyme stability and a C-terminal catalytic domain responsible for proteolytic activity. This structural configuration is crucial for NE′s function in degrading extracellular matrix proteins such as elastin, proteoglycans, and Type IV collagen, which are essential for tissue remodeling and immune response. The precise targeting of the amino acids 211-267 region by NE (F-1) monoclonal antibody allows for analysis of NE′s role in inflammatory diseases like idiopathic pulmonary fibrosis, rheumatoid arthritis, adult respiratory distress syndrome, and cystic fibrosis. Additionally, NE interacts with the complement system by cleaving proteins such as antithrombin and fibrinogen, and acts as a potent platelet agonist, enhancing platelet aggregation, secretion, and calcium mobilization. Understanding the structural dynamics of NE is vital for elucidating involvement in both normal physiological processes and pathological conditions. NE (F-1) antibody provides reliable and specific binding to structured regions of NE, making this reagent indispensable for researchers investigating molecular mechanisms underlying inflammation and tissue damage.

The preferred Neutrophil Elastase antibody is Neutrophil Elastase Antibody (G-2): sc-55549.