Neutrophil Elastase Antibody (D-7): sc-365950

Neutrophil Elastase Antibody (D-7) is a mouse monoclonal IgG1 kappa light chain antibody engineered to recognize an epitope mapping between amino acids 251-267 at the C-terminus of human Neutrophil Elastase (NE), also known as ELANE or medullasin. Anti-Neutrophil Elastase antibody (D-7) reliably detects NE in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA), making this tool essential for research in various settings. NE is a serine protease primarily stored in the azurophilic granules of neutrophils, where NE plays a pivotal role in degrading phagocytosed pathogens and remodeling the extracellular matrix during immune responses. NE interacts with several key proteins, including antithrombin and fibrinogen, mediating proteolysis that influences the complement system and coagulation pathways. Additionally, NE interacts with extracellular matrix proteins such as elastin and Type IV collagen, contributing to tissue remodeling and inflammatory processes. These interactions are critical in the progression of inflammatory diseases like idiopathic pulmonary fibrosis, rheumatoid arthritis, adult respiratory distress syndrome, and cystic fibrosis, where NE contributes to tissue damage and immune dysregulation. By specifically targeting the C-terminal region of NE, Neutrophil Elastase (D-7) antibody provides high specificity and affinity, enabling accurate detection and study of NE′s interactions and functions. Neutrophil Elastase monoclonal antibody (D-7) proves indispensable for researchers investigating the molecular mechanisms of inflammation, immune response, and related pathologies, thereby supporting the development of targeted therapeutic strategies.

The preferred Neutrophil Elastase antibody is Neutrophil Elastase Antibody (G-2): sc-55549.