Nek2 Antibody (D-8) recommended for detection of Nek2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Nek2 Antibody (D-8): sc-55601

Nek2 Antibody (D-8) is rated 4.6 out of 5 by 19.
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Datasheets

    • Anti-Nek2 Antibody (D-8) is a mouse monoclonal IgG2b (kappa light chain) Nek2 antibody provided at 200 µg/ml
    • raised against amino acids 211-445 mapping at the C-terminus of Nek2 of human origin
    • Anti-Nek2 Antibody (D-8) is recommended for detection of Nek2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-Nek2 Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Nek2 (D-8): sc-55601.

Does this antibody work for co-IP?

Asked by: PENDF
Thank you for your question. Yes, this antibody is great for Co-IP. We have several recent publications, here are a few examples: PMID: 30370607, 29863498.
Answered by: Tech Service
Date published: 2019-12-10

I need to use an antibody to detect the protein in samples of equine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2017-02-01
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Rated 1 out of 5 by from Does not work for IHC or IF Tried tw different clones of this antibody, and none of them work for IHC or IF. No staining.
Date published: 2019-04-16
Rated 4 out of 5 by from good product We used 1:1000 dilution to do WB in U2OS whole cell lysate. The signal is very weak, needs long exposure (about 2 hours) to get the signal with some non-specific bands.
Date published: 2018-02-09
Rated 4 out of 5 by from Specific Signal Worked well and specifically on mouse tissues using WB.
Date published: 2018-01-24
Rated 5 out of 5 by from Sehr gute Ergebnisse in der Immunfluoreszenz. Sehr gute Ergebnisse in der Immunfluoreszenz mit humanen und murinen Zelllinien (NIH, hTERT RPE-1). Es wurde die Verdünnung 1:500 benutzt.
Date published: 2018-01-04
Rated 5 out of 5 by from I feel fine A few days ago our lab used this antibody, feeling good results. I'm considering buying more
Date published: 2017-06-02
Rated 5 out of 5 by from satisfied with the antibody We used it to detect cancer cell protein, get the desired results, about 47 kDa, satisfied with the antibody.
Date published: 2017-06-01
Rated 5 out of 5 by from positive signal Good antibody for IF paraffin-embedded human lung tissue,positive signal.
Date published: 2017-04-15
Rated 5 out of 5 by from recommend I found that Western Blotting can usually be diluted with 1:500,recommend to everyone.
Date published: 2017-02-25
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