MUP Antibody (F-3): sc-374075

MUP Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects MUP protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MUP (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Major urinary proteins (MUPs) serve as pheromone-binding proteins that are excreted in urine, playing a crucial role in influencing physiological and behavioral responses in mammals. These proteins are primarily synthesized in the liver and several secretory tissues, such as the lachrymal, submaxillary, and mammary glands, highlighting their importance in various biological processes. MUPs are products of a multi-gene family that exhibit diverse tissue-specific expression, developmental regulation, and hormonal control, with several isoforms being expressed and secreted predominantly by sexually mature male mice. The structure of MUP proteins is characterized by a beta-barrel motif, which is essential for accommodating a broad range of pheromones, thereby facilitating their binding and subsequent signaling. Notably, testosterone treatment can significantly influence the MUP phenotype by enhancing MUP excretion and altering the relative proportions of each isoform, suggesting that MUPs may play a vital role in mediating olfactory cues through differential pheromone-binding profiles.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MUP Antibody (F-3) References:

1. Expression of major urinary protein genes in the nasal glands associated with general olfaction.  |  Utsumi, M., et al. 1999. J Neurobiol. 39: 227-36. PMID: 10235677
2. Solution structure of a recombinant mouse major urinary protein.  |  Lücke, C., et al. 1999. Eur J Biochem. 266: 1210-8. PMID: 10583419
3. Structural basis of pheromone binding to mouse major urinary protein (MUP-I).  |  Timm, DE., et al. 2001. Protein Sci. 10: 997-1004. PMID: 11316880
4. The major urinary protein system in the rat.  |  Gómez-Baena, G., et al. 2014. Biochem Soc Trans. 42: 886-92. PMID: 25109974
5. Molecular complexity of the major urinary protein system of the Norway rat, Rattus norvegicus.  |  Gómez-Baena, G., et al. 2019. Sci Rep. 9: 10757. PMID: 31341188
6. Major urinary protein excreted in rodent hindpaw sweat.  |  Poitras, T., et al. 2021. J Anat. 239: 529-535. PMID: 33686663
7. Genetically determined electrophoretic variants of the major urinary protein (Mup) complex in mouse urine.  |  Groen, A. and Lagerwerf, AJ. 1979. Anim Blood Groups Biochem Genet. 10: 107-14. PMID: 507473
8. Structure of mouse major urinary protein genes: different splicing configurations in the 3'-non-coding region.  |  Clark, AJ., et al. 1984. EMBO J. 3: 1045-52. PMID: 6329733
9. Subfamilies of the mouse major urinary protein (MUP) multi-gene family: sequence analysis of cDNA clones and differential regulation in the liver.  |  Kuhn, NJ., et al. 1984. Nucleic Acids Res. 12: 6073-90. PMID: 6548015
10. Tissue-specific expression of major urinary protein (MUP) genes in mice: characterization of MUP mRNAs by restriction mapping of cDNA and by in vitro translation.  |  Shahan, K. and Derman, E. 1984. Mol Cell Biol. 4: 2259-65. PMID: 6549044
11. Regulation of mouse major urinary protein production by the Mup-A gene.  |  Szoka, PR. and Paigen, K. 1978. Genetics. 90: 597-612. PMID: 730052
12. Protein-bound male urinary pheromones: differential responses according to age and gender.  |  Mucignat-Caretta, C., et al. 1998. Chem Senses. 23: 67-70. PMID: 9530971

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MUP Antibody (F-3) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-MUP Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MUP Antibody (F-3) Specifications

Alternate Product Name: MUP

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-181

Epitope Target: MUP

Epitope Species: rat

Product Reactivity/Detection: a broad range of MUP proteins

Species Reactivity/Detection: mouse, rat

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for MUP Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred MUP antibody is MUP Antibody (C-7): sc-166429.