MHC class II Antibody (12F4): sc-53896
- MHC class II Antibody (12F4) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
- raised against stroma cells of human origin
- recommended for detection of MHC class II of human origin by IF
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for MHC class II Antibody (12F4). These reagents are now offered in bundles with MHC class II Antibody (12F4) (see ordering information below).
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MHC class II Antibody (12F4) is a mouse monoclonal IgG1 antibody that detects MHC class II in human samples through immunofluorescence (IF) applications. MHC class II molecules play a crucial role in the immune response by presenting processed exogenous antigens to helper T lymphocytes, which are essential for orchestrating adaptive immunity. These molecules are primarily located on the surface of professional antigen-presenting cells, such as dendritic cells, macrophages, and B cells, where they facilitate the activation of CD4+ T cells. MHC class II (12F4) antibody recognizes longer antigenic peptides, typically ranging from 13 to 18 amino acids, which is vital for effective immune recognition and response. This structural characteristic allows for a diverse range of peptide binding, which is essential for the immune system′s ability to recognize and respond to a wide variety of pathogens. The interaction between MHC class II and T cell receptors is a key event in the activation of the adaptive immune response, making anti-MHC class II antibody (12F4) an invaluable tool for studying immune function and the mechanisms of immune-mediated diseases.
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MHC class II Antibody (12F4) References:
- Proteases involved in MHC class II antigen presentation. | Villadangos, JA., et al. 1999. Immunol Rev. 172: 109-20. PMID: 10631941
- MHC class II molecules traffic into lipid rafts during intracellular transport. | Poloso, NJ., et al. 2004. J Immunol. 173: 4539-46. PMID: 15383586
- Ii-Key/MHC class II epitope hybrids: a strategy that enhances MHC class II epitope loading to create more potent peptide vaccines. | Kallinteris, NL., et al. 2006. Expert Opin Biol Ther. 6: 1311-21. PMID: 17223739
- Measurement of Peptide Binding to MHC Class II Molecules by Fluorescence Polarization. | Yin, L. and Stern, LJ. 2014. Curr Protoc Immunol. 106: 5.10.1-5.10.12. PMID: 25081912
- Exogenous antigens bind MHC class II first, and are processed by cathepsins later. | Sadegh-Nasseri, S. and Kim, A. 2015. Mol Immunol. 68: 81-4. PMID: 26254987
- MHC class II expression by the gut epithelium. | Bland, P. 1988. Immunol Today. 9: 174-8. PMID: 3151441
- A Missing Switch in Peptide Exchange for MHC Class II Molecules. | Freund, C. and Höfer, T. 2019. Front Immunol. 10: 2513. PMID: 31708929
- MHC class II signaling in B-cell activation. | Scholl, PR. and Geha, RS. 1994. Immunol Today. 15: 418-22. PMID: 7524519
- Interactions between staphylococcal superantigens and MHC class II molecules. | Labrecque, N., et al. 1993. Semin Immunol. 5: 23-32. PMID: 8467091
- Functional significance of polymorphism among MHC class II gene promoters. | Guardiola, J., et al. 1996. Tissue Antigens. 48: 615-25. PMID: 9008303
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MHC class II Antibody (12F4) | sc-53896 | 200 µg/ml | $322.00 | |||
MHC class II Antibody (12F4): m-IgG Fc BP-HRP Bundle | sc-537012 | 200 µg Ab; 10 µg BP | $361.00 | |||
MHC class II Antibody (12F4): m-IgGκ BP-HRP Bundle | sc-534192 | 200 µg Ab; 40 µg BP | $361.00 | |||
MHC class II Antibody (12F4): m-IgG1 BP-HRP Bundle | sc-544955 | 200 µg Ab; 20 µg BP | $361.00 |