MHC class II Antibody (IBL-5/22) recommended for detection of MHC class II of mouse origin by WB, IP, IF and FCM

MHC class II Antibody (IBL-5/22): sc-59322

MHC class II Antibody (IBL-5/22) is rated 4.5 out of 5 by 8.
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Datasheets

    • MHC class II Antibody (IBL-5/22) is a rat monoclonal IgG1 provided at 100 µg/ml
    • raised against spleen cells of mouse origin
    • recommended for detection of MHC class II of mouse origin by WB, IP, IF and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of MHC class II (IBL-5/22): sc-59322.
Submit a review for this product and receive 15 CruzCredits

which lysis buffer would be best to use while doing immunoprecipitation with this antibody?

Asked by: gagan
Thank you for your question. We recommend RIPA buffer. Here is a link to our IP protocol for your convenience: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Tech Service
Date published: 2019-04-22

what is the immunogen for sc 59322

Asked by: Bertie
Thank you for your question. This mouse monoclonal antibody was raised against spleen cells of mouse origin. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-03-04

What forms of MHC-II does this antibody recognise?

Asked by: lympho
Thank you for your question. Unfortunately we do not have this information. The exact epitope for this antibody has not been mapped, so no homology check can be performed either. Please contact Technical Service if you have any further questions.
Answered by: Tech Service
Date published: 2019-01-17

Does MHC class II Antibody (IBL-5/22): sc-59322 react with human samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 15
Date published: 2017-01-22
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Rated 5 out of 5 by from Great antibody for IF and FC Great staining in both IF and FC using B lymphocytes (mouse cell line). Dilution 1:50. It works both before and after 4% PFA fixation.
Date published: 2019-01-10
Rated 5 out of 5 by from Strong WB Very nice western blot data; strong signal, low background
Date published: 2017-02-02
Rated 5 out of 5 by from Great Antibody! This antibody works great in western blot analysis of MHC class II expression in mouse PBL whole cell lysate.
Date published: 2017-01-11
Rated 1 out of 5 by from No bands observed with antibody for mouse No bands observed with antibody for mouse spleen lysate.
Date published: 2015-03-26
Rated 5 out of 5 by from Good antibody data for mouse splenocytes Good antibody data for mouse splenocytes using WB and IP. -SCBT Publication Review
Date published: 2015-03-18
Rated 5 out of 5 by from Good immunocytochemistry data in mouse neural Good immunocytochemistry data in mouse neural progenitor cells and microglia. -SCBT Publication Review
Date published: 2015-02-05
Rated 5 out of 5 by from Antibody works well for WB in C57BL/6 mice Antibody works well for WB in C57BL/6 mice peritonial macrophages. -SCBT Publication Review
Date published: 2015-01-22
Rated 5 out of 5 by from Works well for WB in mouse PBL whole cell lysate Works well for WB in mouse PBL whole cell lysate. -SCBT QC
Date published: 2014-10-16
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