MDR1/ABCB1 Antibody (D-11): sc-55510

MDR1 Antibody (D-11) is a mouse monoclonal IgG2b kappa light chain antibody engineered to target the human MDR1 protein, also known as ABCB1, by recognizing the amino acids 1040-1280 epitope. MDR1 monoclonal antibody (D-11) effectively detects MDR1 across multiple species, including mouse, rat, and human, utilizing techniques such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA), and may also cross-react with the mouse homolog Mdr-3. The MDR1 protein, also referred to as ABCB1, P-GP, PGY1, ABC20, CD243, ENPAT, GP170, and p-170, is a crucial member of the ATP-binding cassette (ABC) transporter family, residing primarily in the apical membrane of cells within the blood-brain barrier, liver, kidneys, and intestines. The primary function of MDR1 is to act as a drug-transport pump, actively effluxing a diverse array of chemotherapeutic agents and other xenobiotics out of cells, which plays a significant role in the development of multiple drug resistance (MDR) in cancer therapy. By maintaining the protective barrier of the blood-brain barrier, MDR1 prevents the accumulation of potentially harmful substances in the brain, thereby safeguarding neural tissue. Understanding and accurately detecting MDR1 is essential for researchers focused on elucidating mechanisms of drug resistance and for the development of strategies to overcome therapeutic challenges in various diseases. Anti-MDR1 antibody (D-11) is available in both non-conjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and a range of Alexa Fluor® conjugates, providing versatile options for diverse experimental applications. By facilitating detailed studies of MDR1′s cellular localization and function, MDR1 (D-11) antibody serves as an indispensable tool for advancing research in pharmacology, oncology, and neurobiology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MDR1/ABCB1 Antibody (D-11) References:

1. Down-regulation of ras and myc expression associated with mdr-1 overexpression in adriamycin-resistant tumor cells.  |  Banerjee, S., et al. 1992. Cell Mol Biol. 38: 561-70. PMID: 1363885
2. [MDR1 genotypes related to pharmacokinetics and MDR1 expression].  |  Nakamura, T. 2003. Yakugaku Zasshi. 123: 773-9. PMID: 14513768
3. Modulation of MDR-1 gene by MIF and GSTpi with drug resistance generation in hormone independent prostate cancer.  |  Yu, DS., et al. 2006. Arch Androl. 52: 283-91. PMID: 16728344
4. Cyp3A4, Cyp3A5, and MDR-1 genetic influences on tacrolimus pharmacokinetics in renal transplant recipients.  |  Roy, JN., et al. 2006. Pharmacogenet Genomics. 16: 659-65. PMID: 16906020
5. [Is MDR 1 gene a key to successful chemotherapy?].  |  Wilczyński, JR., et al. 2006. Ginekol Pol. 77: 476-84. PMID: 16964701
6. Nkx-2.5 Regulates MDR1 Expression via Its Upstream Promoter in Breast Cancer Cells.  |  Lim, JS., et al. 2019. J Korean Med Sci. 34: e100. PMID: 30940996
7. Hedgehog-Gli2 Signaling Promotes Chemoresistance in Ovarian Cancer Cells by Regulating MDR1.  |  Wang, Q., et al. 2021. Front Oncol. 11: 794959. PMID: 35059317
8. Generation of a drug resistance profile by quantitation of mdr-1/P-glycoprotein in the cell lines of the National Cancer Institute Anticancer Drug Screen.  |  Alvarez, M., et al. 1995. J Clin Invest. 95: 2205-14. PMID: 7738186
9. MDR-related P170-glycoprotein modulates cytotoxic activity of homoharringtonine.  |  Russo, D., et al. 1995. Leukemia. 9: 513-6. PMID: 7885049
10. P-glycoprotein (MDR 1 gene product) in cells of the immune system: its possible physiologic role and alteration in aging and human immunodeficiency virus-1 (HIV-1) infection.  |  Gupta, S. and Gollapudi, S. 1993. J Clin Immunol. 13: 289-301. PMID: 7902361
11. The human multidrug resistance-associated protein MRP is a plasma membrane drug-efflux pump.  |  Zaman, GJ., et al. 1994. Proc Natl Acad Sci U S A. 91: 8822-6. PMID: 7916458
12. Analysis of the expression of MRP, the gene for a new putative transmembrane drug transporter, in human multidrug resistant lung cancer cell lines.  |  Zaman, GJ., et al. 1993. Cancer Res. 53: 1747-50. PMID: 8467491

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MDR1/ABCB1 Antibody (D-11) has 189 citations in a variety of scientific publications.

Primary antibodies like Anti-MDR1/ABCB1 Antibody (D-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MDR1/ABCB1 Antibody (D-11) Specifications

Alternate Product Name: MDR1

Product Clone Name: D-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1040-1280

Epitope Target: MDR1

Epitope Species: human

Product Reactivity/Detection: MDR1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 189 citations in various scientific publications

Available Conjugates/Forms for MDR1/ABCB1 Antibody (D-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin