LIME Antibody (E-1): sc-166818

LIME Antibody (E-1) is a mouse monoclonal IgG1 antibody that detects LIME in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LIME, or Lck-interacting molecule, is a 295 amino acid transmembrane adaptor protein predominantly expressed in hematopoietic and lung cells, featuring a short extracellular domain and a cytoplasmic tail rich in five tyrosine-based motifs. Upon cross-linking of the CD4 or CD8 co-receptors, LIME undergoes tyrosine phosphorylation, which is crucial for its interaction with Lck, a member of the Src family kinases, and Csk, its negative regulator. This phosphorylation event is significant as LIME plays a vital role in the regulation of T cell activation, particularly during the early and late stages of this process. Furthermore, LIME is implicated in the activation of key signaling pathways, including the ERK and JNK pathways, which are essential for T cell function and response. Additionally, LIME may also participate in B cell receptor-mediated B cell activation, highlighting LIME′s importance in the immune response. Anti-LIME antibody (E-1) is an invaluable tool for researchers studying T cell activation and signaling pathways in human cells.

The preferred LIME antibody is LIME Antibody (A-7): sc-365195.